16658

5-溴-4-氯-3-吲哚基-N-乙酰-β-D-氨基半乳糖苷

reagent for selection of recombinant bacterial clones

• 同義詞: 5-溴-4-氯-3-吲哚基-N-乙酰氨基-2-脱氧-β-D-吡喃半乳糖苷, X-GalNAc
• 經驗公式（希爾表示法）: C₁₆H₁₈BrClN₂O₆
• CAS號碼: 129572-48-1
• 分子量：: 449.68
• MDL號碼: MFCD00083260
• 分類程式碼代碼: 12352200
• PubChem物質ID: 329751315

屬性

• 等級: for molecular biology
• 無菌: non-sterile
• 化驗: ≥98.0% (TLC)
• 形狀: powder
• mp: ≥220 °C (lit.)
• 溶解度: DMF: soluble; DMSO: soluble
• 適合性: suitable for β-galactosidase test
• 儲存溫度: −20°C
• SMILES 字串: CC(=O)N[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1Oc2c[nH]c3ccc(Br)c(Cl)c23
• InChI: 1S/C16H18BrClN2O6/c1-6(22)20-13-15(24)14(23)10(5-21)26-16(13)25-9-4-19-8-3-2-7(17)12(18)11(8)9/h2-4,10,13-16,19,21,23-24H,5H2,1H3,(H,20,22)/t10-,13-,14+,15-,16-/m1/s1
• InChI 密鑰: SUWPNTKTZYIFQT-LMXXTMHSSA-N

描述

一般說明

5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide (X-GalNAc)is a chromogenic substrate for for N-acetyl-β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates.

應用

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for the detection of Candida albicans.

原則

When X-GalNAc is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GalNAc turn blue in color, allowing for easy differentiation between lac+ and lac- colonies.

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)