16669

Magenta-Gal

reagent for selection of recombinant bacterial clones

• 同義詞: 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal
• 經驗公式（希爾表示法）: C₁₄H₁₅BrClNO₆
• CAS號碼: 93863-88-8
• 分子量：: 408.63
• Beilstein: 1552602
• MDL號碼: MFCD00210022
• 分類程式碼代碼: 12352200
• PubChem物質ID: 329751317

屬性

• 等級: for molecular biology
• 品質等級: 100
• 無菌: non-sterile
• 化驗: ≥98.0% (HPLC)
• 形狀: powder
• 溶解度: DMF: soluble; DMSO: soluble
• 適合性: suitable for β-galactosidase test
• 儲存溫度: −20°C
• SMILES 字串: OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1
• InChI 密鑰: CHRVKCMQIZYLNM-RKQHYHRCSA-N

描述

一般說明

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

應用

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

原則

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)