SCT275

BioTracker^™ Green Late-Phase Autophagy Live Cell Probe

Human

• 同義詞: autolysosome live cell probe, green autophagy probe
• 分類程式碼代碼: 41106514
• NACRES: NA.47

屬性

• 產品名稱: BioTracker^™ Green Late-Phase Autophagy Live Cell Probe,
• 生物源: human
• 品質等級: 100
• 包裝: vial of 20 nmol
• 製造商／商標名: Millipore
• 技術: cell culture | stem cell: suitable; flow cytometry: suitable
• 運輸包裝: 2-8°C
• 儲存溫度: 2-8°C

描述

一般說明

SCT275 is used to detect late phase autophagy in live cells. This small hydrophobic probe molecule is plasma membrane-permeable and incorporates in to the autophagosome. Following lysosome-autophagosome fusion, increased acidity in the autolysosome enhances fluorescence signal of the BioTracker^™ Green Late-Phase Autophagy Live Cell Probe.SPECTRAL CHARACTERISTICS: Excitation λ: 405 nmEmission λ: 525 nmRecommended laser/filter:Ex 350 - 450 nmEm 500 nm - 560 nm

特點和優勢

Fluorescence microscopyFlow cytometry

標靶描述

Autophagy is an intracellular degradation process, where dysfunctional proteins and organelles are degraded by a system of organelles. Aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. DALGreen, which is a small hydrophobic molecule, passes the plasma membrane of live cells and is incorporated in the autophagosome.

儲存和穩定性

Store at -20 °C, protected from light.

其他說明

SCT275 is a small hydrophobic molecule live cell probe that localizes to the autolysosome. The probe fluoresces green as the autolysosome environment acidifies, enabling the assessment of autophagy by fluorescence microscopy and flow cytometry.Add 20 uL DMSO to tube of SCT275 and pipet to dissolve to make a 1 mM stock solution. Dilute with culture medium to 0.1 - 1.0 uM to make working solution.

法律資訊

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable