The concentration provided at 200uM in DMSO solution, final volume 50ul. Please spin down this material before usage to be able to collect all the solution in the bottom of the tube. It is recommended to use the material at 1:1000 dilution, but this is sample dependent.
推薦產品
分子量
observed mol wt 1218.5 kDa
品質等級
包裝
vial of 50 μL
製造商／商標名
Millipore
增長模式
N/A
技術
cell based assay: suitable
flow cytometry: suitable
檢測方法
fluorometric
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
The BioTracker™ Membright-488 live cell dye is a “turn-on” fluorescence probe emitting in the far red spectrum for the imaging of plasma membranes of live cells.
Specific plasma membrane markers are essential for delineating the cell. Membright dyes exploit a unique fluorogenic mechanism, existing in a self-quenched nanoparticle state. Fluorescence is turned on upon contact with and propagation within the plasma membrane, producing bright, background-free signal. The Membright live cell membrane dyes are compatible with two-photon imaging, superresolution imaging, and fixed cell preparations. The high photostability and lack of cytotoxicity of Membright dyes make them suitable for long-term live cell imaging. The suite of superior characteristics of Membright plasma membrane dyes permit use in a wide spectrum of fluorescence microscopy applications and flow cytometry.
Spectral Properties
Fluorescence images obtained by λex = 488 nm and emission range of 500-550 nm.
Reference
Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614
Specific plasma membrane markers are essential for delineating the cell. Membright dyes exploit a unique fluorogenic mechanism, existing in a self-quenched nanoparticle state. Fluorescence is turned on upon contact with and propagation within the plasma membrane, producing bright, background-free signal. The Membright live cell membrane dyes are compatible with two-photon imaging, superresolution imaging, and fixed cell preparations. The high photostability and lack of cytotoxicity of Membright dyes make them suitable for long-term live cell imaging. The suite of superior characteristics of Membright plasma membrane dyes permit use in a wide spectrum of fluorescence microscopy applications and flow cytometry.
Spectral Properties
Fluorescence images obtained by λex = 488 nm and emission range of 500-550 nm.
Reference
Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614
應用
Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.
特點和優勢
Green fluorescent probe for efficient, selective labeling of the plasma membrane in live cells. Suitable for superresolution microscopy.
外觀
Green solution
儲存和穩定性
Store long term at -20°C, protected from light.
其他說明
Live cell fluorescent imaging, flow cytometry
法律資訊
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
188.6 °F - closed cup
閃點（°C）
87 °C - closed cup
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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What is the concentration of membright 488. Only info I have is that it is 50 microlitres. Publications use it at 20 nM
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