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NA81

Sigma-Aldrich

5-甲基胞嘧啶小鼠单克隆抗体(162 33 D3)

liquid, clone 162 33 D3, Calbiochem®

同義詞:

抗-5-mc、抗-5-MeCyd

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

162 33 D3, monoclonal

形狀

liquid

不包含

preservative

物種活性(以同源性預測)

all

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG1

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

免疫亲和纯化的小鼠单克隆抗体。识别5-甲基胞嘧啶。
经验证,该抗5-甲基胞嘧啶小鼠mAb(162 33 D3)可用于ELISA、FC、冷冻切片、IF、石蜡切片、放射免疫分析、DNA-蛋白质印迹法检测5-甲基胞嘧啶。
识别NIH3T3细胞中甲基化DNA或RNA中的5-甲基胞嘧啶。

特异性
亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
流式细胞术
冷冻切片
免疫印迹
免疫荧光
石蜡切片

可靠
产生可重复的结果。
克隆162 33 D3已在50多个出版物中使用。

免疫原

5-甲基胞嘧啶结合卵清蛋白

應用



ELISA(0.1 g/mL)
流式细胞术(1 g/mL)
冷冻切片(5-10 g/mL)
免疫印迹法(5 g/mL)
免疫荧光(1 g/mL)
石蜡切片(5-10 g/mL)
放射免疫分析(0.1 g/mL)
DNA-蛋白质印迹法(5 g/mL)

包裝

批次特定浓度请参见小瓶标签。

警告

毒性:标准处理(A)

外觀

在PBS(pH 7.4)中。

重構

初次解冻后,等分并冷冻(-20°C)。

分析報告

阳性对照
NIH3T3细胞

其他說明

Ehrlich, M. 2002.Oncogene21, 5400.(Review)
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
克隆162 33 D3可用于定量和定性检测各种样品和应用中的甲基化DNA或RNA。它已在40多个出版物中使用。应滴定抗体,以在单个系统中获得最佳结果。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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