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MCYTOMAG-70K-PMX

Millipore

MILLIPLEX®小鼠细胞因子/趋化因子磁珠试剂盒 - 预混25重 - 免疫学多重分析

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

同義詞:

Luminex® Mouse cytokine immunoassay, Millipore Mouse cytokine immunoassay, Mouse cytokine Multiplex Immunoassay

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.47
暫時無法取得訂價和供貨情況

品質等級

200

物種活性

mouse

製造商／商標名

Milliplex®

assay range

accuracy: 85-107%
standard curve range: 3.2-10,000 pg/mL

技術

multiplexing: suitable

相容性

configured for Premixed

檢測方法

fluorometric (Luminex xMAP)

運輸包裝

wet ice

一般說明

为了鉴别参与任何炎症或免疫反应的特定细胞因子，可能有必要筛选细胞因子组，通常需要一定水平的自动化和/或高通量。微珠可以使自动化和高通量筛选过程更容易，并具有自动化洗涤等功能。甚至在自动化之外的优势包括:

  • 更柔韧的板和洗板机选择
  • 使用浑浊的血清/血浆样品改善性能
  • 测定结果等同于非微珠
  • 自动洗涤避免了与真空过滤洗涤相关的许多问题


MILLIPLEX®小鼠细胞因子/趋化因子组套使您能够专注于细胞因子的治疗潜力以及细胞因子表达的调节。结合微珠格式的Luminex ® xMAP®平台，您可以获得理想的速度和灵敏度的优势，允许同时对数十种分析物进行定量多重检测，从而可以显著提高生产力。

组套类型：细胞因子/趋化因子

應用

  • 分析物：G-CSF, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, MCP-1, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α

其他說明

灵敏度：有关单个细胞因子的灵敏度，请参阅试剂盒方案。

法律資訊

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

標靶器官

Respiratory Tract

儲存類別代碼

10 - Combustible liquids

分析證明 (COA)

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Dana M Hardbower et al.
Amino acids, 48(10), 2375-2388 (2016-04-15)
We reported that arginase 2 (ARG2) deletion results in increased gastritis and decreased bacterial burden during Helicobacter pylori infection in mice. Our studies implicated a potential role for inducible nitric oxide (NO) synthase (NOS2), as Arg2 (-/-) mice exhibited increased
Elena A Govorkova et al.
Antimicrobial agents and chemotherapy, 59(3), 1495-1504 (2014-12-24)
Compounds that target the cellular factors essential for influenza virus replication represent an innovative approach to antiviral therapy. Sp2CBMTD is a genetically engineered multivalent protein that masks sialic acid-containing cellular receptors on the respiratory epithelium, which are recognized by influenza
Kshipra Singh et al.
American journal of physiology. Gastrointestinal and liver physiology, 305(3), G225-G240 (2013-05-25)
L-Arginine (L-Arg) is a semiessential amino acid that has altered availability in human ulcerative colitis (UC), a form of inflammatory bowel disease, and is beneficial in murine colitis induced by dextran sulfate sodium (DSS), a model with similarity to UC.
Wei Lei et al.
PloS one, 11(8), e0160994-e0160994 (2016-08-31)
Sutherlandia frutescens is a medicinal plant that has been traditionally used in southern Africa for cancers, infections, and inflammatory conditions. We recently published experiments demonstrating that an aqueous extract of S. frutescens possessed potent immune-stimulatory activity. This work was carried
Rubia I Mancuso et al.
Immunity, inflammation and disease, 6(1), 128-142 (2017-11-10)
Streptococcus pneumoniae colonizes the nasopharynx of healthy individuals establishing a commensal relationship with the host. In some conditions, bacteria invade the lower respiratory tract and innate immune responses are crucial to avoid diseases such as pneumonia, sepsis, or meningitis. Here
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Questions

1–2 of 2 Questions  

  1. It seems that the sample wells are experiencing low bead counts while the standard and control wells are unaffected. This issue is limited to 1 or 2 cytokines, while the rest show normal counts. It doesn't appear to be related to the instrument, bead loss, or inadequate bead mixing. What could be the possible reason for this discrepancy?

    1 answer

    1. To reduce the occurrence of sample-related bead aggregation, the following steps can be taken:
      •Spin samples at 10,000 x g for 10 minutes immediately before preparing sample dilutions or loading samples into the plate.
      •Resuspend the plate in Wash Buffer instead of Sheath or Drive Fluid. The plate must be read within a couple of hours, and the buffer should be switched to Sheath or Drive Fluid for longer storage.
      •Dilute samples at a ratio of 1:2 or 1:4.
      •Perform more wash cycles after the primary incubation step of the assay to remove substances that cause bead aggregation.

      Helpful?

  2. Does the addition of 25 µL of Assay Buffer to the sample well count as a dilution when using a Milliplex kit that does not require a sample dilution prior to loading the plate?

    1 answer

    1. If the sample is not diluted prior to loading the plate, and the same volume of standard and sample are loaded, then there is no need to apply a sample dilution factor.

      Helpful?

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