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Merck
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重要文件

MABT456

Sigma-Aldrich

Anti-MSN Antibody, clone 2C12

ascites fluid, clone 2C12, from mouse

同義詞:

Moesin, Membrane-organizing extension spike protein

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

2C12, monoclonal

物種活性

human

技術

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

同型

IgG1

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... MSN(4478)

一般說明

Moesin (membrane-organizing extension spike protein) is the protein encoded by the MSN gene. The MSN encoded protein moesin is a member of the ERM (ezrin, moesin, radixin) family of proteins that regulate linkage between the plasma membrane and the actin cytoskeleton. Thus, the MSN encoded protein is a peripheral membrane protein that is keenly involved in the signaling and structural connections in cytoskeletal plasma membrane structures like microvilli. Moesin in its active, phosphorylated state strongly binds actin and helps anchor it to the plasma membrane. The MSN encoded Moesin is localized to filopodia and other membranous extensions of various cell types and thus plays a role in cell-cell recognition signaling, cell movement, and cell transport. In its inactive, non-phosphorylated state, Moesin binds itself in a head-tail fashion and is incapable of binding actin or other cytoskeletal proteins. The kinase ROCK2 is central to the opening up of moesin and allowing its interaction with actin and the plasma membrane. EMD-Millipore’s Anti-MSN, clone 2C12 has been tested in western blots against purified recombinant protein as well as multiple human cancer cell lysates. The clone has also been tested successfully in paraffin embedded immunohistochemistry on human colon tissue and by flow cytometry on human Jurkat cells and ELISA using purified recombinant antigen.

免疫原

Purified protein fragment of human MSN expressed in E. coli.

應用

Detect Moesin using this mouse monoclonal antibody, Anti-MSN Antibody, clone 2C12 validated for use in western blotting, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MSN in human tonsil and colon tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MSN in Jurkat cells.

Optimal working dilutions must be determined by end user.

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MSN in 10 µg of Jurkat cell lysate.

標靶描述

~68 kDa observed. Uncharacterized bands may appear in some lysate(s).

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象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Aquatic Chronic 3 - Repr. 1A

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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