MABE936

Anti-ASF-1 Antibody, clone AK-96

clone AK-96, from mouse

• 同義詞: Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: AK-96, monoclonal
• 物種活性: human
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_001071634
• UniProt登錄號: Q07955
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SRSF1(6426)

描述

一般說明

Alternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

免疫原

Recombinant protein corresponding to human ASF-1.

應用

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-ASF-1 Antibody, clone AK-96 is validated for use in western blotting, IP & ICC for the detection of ASF-1.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of Raji and HepG2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected ASF-1 in BJ fibroblast cells (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 in BJ fibroblast cell lysate (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of HeLa cell lysate.

標靶描述

~30 kDa observed

聯結

Replaces: MABE512

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable