MABC1040

Anti-MTH1 Antibody, clone 11A3.2

clone 11A3.2, from mouse

• 同義詞: 7,8-dihydro-8-oxoguanine triphosphatase, 2-hydroxy-dATP diphosphatase, 8-oxo-dGTPase, Nucleoside diphosphate-linked moiety X motif 1, Nudix motif 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 11A3.2, monoclonal
• 物種活性: human
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_002443
• UniProt登錄號: P36639
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NUDT1(4521)

描述

一般說明

7,8-dihydro-8-oxoguanine triphosphatase (EC 3.6.1.55; UniProt P36639; also known as 2-hydroxy-dATP diphosphatase, 8-oxo-dGTPase, Nucleoside diphosphate-linked moiety X motif 1, Nudix motif 1) is encoded by the NUDT1 (also known as MTH1) gene (Gene ID 4521) in human. Human MTH1/NUDT1 is the bacterial mutT ortholog that functions as a nucleotide pool-sanitising enzyme. MTH1/NUDT1 cleaves oxidized nucleotides such as 8-oxo-deoxyguanosinetri-phosphate (8-oxo-dGTP) and converts the triphosphates into the corresponding mono-phosphates. The hydrolysis reaction assures that the oxidized nucleotides can no longer be recognized by DNA polymerase, thereby preventing bases mispairing and transversion mutations by avoiding their incorporation during DNA replication. Upregulated MTH1 levels are found in cancer cells as a means to reduce 8-oxo-dGTP accumulation in the nucleotide pool due to high oxidative stress levels in these cells.

特異性

Clone 11A3.2 recognizes an epitope present in all four spliced isoforms of human NUDT1/MTH1 reported by UniProt (P36639).

免疫原

Epitope: C-terminal half.

GST-tagged recombinant human MTH1 C-terminal fragment.

應用

Anti-MTH1 Antibody, clone 11A3.2 is an antibody against MTH1 for use in Western Blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MTH1 in 10 µg of Jurkat cell lysate.

標靶描述

~20 kDa observed. 22.52 kDa (isoform p26), 20.30 kDa (isoform p22), 19.47 kDa (isoform p21), 17.95 kDa (isoform p18) calculated. Uncharacterized band(s) may appear in some lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable