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重要文件

MAB341

Sigma-Aldrich

抗GABA A受体β2,3链抗体,克隆BD17

clone BD17, Chemicon®, from mouse

同義詞:

GABA(A) receptor subunit beta-2, gamma-aminobutyric acid (GABA) A receptor, beta 2, gamma-aminobutyric acid A receptor, beta 2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
無性繁殖:
BD17, monoclonal
application:
IHC
IP
WB
物種活性:
mouse, feline, monkey, bovine
技術:
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
66
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生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

BD17, monoclonal

物種活性

mouse, feline, monkey, bovine

物種活性(以同源性預測)

rat, human

製造商/商標名

Chemicon®

技術

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG1

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... GABRB2(2561)

一般說明

γ-氨基丁酸A型(GABAA)受体是神经递质配体门控离子通道的成员,该通道介导神经元抑制结合GABA。GABA对GABAA受体的作用受到一系列重要治疗药物的调节,包括巴比妥类,麻醉药和苯二氮卓类药物。

特異性

与GABAA受体的β-链反应。

免疫原

来自牛皮质的纯化GABA/苯二氮受体
表位:GABAA受体β2&β3亚基的胞外域

應用

使用经验证可用于IH、IP &WB的抗GABA A受体β2、3链抗体(克隆BD17)检测GABA A受体β2。
免疫组化:
一个浓度为10-20 μg/mL的先前批次产品已被用于IH中。

免疫沉淀:
一个先前批次该抗体已被用于IP中。

蛋白质印迹:
5-20 µg/mL。识别对应于GABAA受体β链的55 kDa条带。

最佳工作稀释度必须由最终用户确定。

品質

通过蛋白质印迹对小鼠大脑膜裂解物进行评估。

蛋白质印迹:
该抗体的1:500稀释液在10μg小鼠脑膜裂解物上检测到GABA A受体β2,3链。

標靶描述

55kda

聯結

替代品:AB5952

外觀

形式:纯化
纯化的小鼠单克隆IgG1液体,溶于含有10 mM磷酸钾缓冲液pH值7.4和150 mM NaCl的缓冲液中

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Human ?1?3?2L gamma-aminobutyric acid type A receptors: High-level production and purification in a functional state.
Dostalova, Z; Zhou, X; Liu, A; Zhang, X; Zhang, Y; Desai, R; Forman, SA; Miller, KW
Protein Science null
Daniel C Adusei et al.
Neuropharmacology, 59(3), 167-171 (2010-05-18)
Fragile X syndrome is the most common heritable form of mental retardation. It is caused by silencing of the Fmr1 gene and the absence of the encoded protein. The purpose of this study was to examine global protein expression levels
Gamma-aminobutyric acid (GABA) is an autocrine excitatory transmitter in human pancreatic beta-cells.
Braun, M; Ramracheya, R; Bengtsson, M; Clark, A; Walker, JN; Johnson, PR; Rorsman, P
Diabetes null
Mirko Feldmann et al.
Cerebral cortex (New York, N.Y. : 1991), 29(2), 892-905 (2018-12-12)
Although by adulthood cortical structures and their capacity for processing sensory information have become established and stabilized, under conditions of cortical injury, or sensory deprivation, rapid reorganization occurs. Little is known as to the impact of this kind of adaptation
Pengju Ma et al.
International journal of clinical and experimental medicine, 8(8), 14296-14302 (2015-11-10)
This study aimed to investigate GABA effects on blood pressure and blood dynamics of anesthetic rats by observing spontaneously hypertensive rats under both anesthesia and waking state. 72 male waking Wistar-Kyokos (WKY) rats and 72 male anesthetized spontaneously hypertensive (SHR)

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