GF021

Stem Cell Factor Protein, Recombinant human

The Stem Cell Factor (SCF) Protein, Recombinant human, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.

• 同義詞: SCF, Mast Cell Growth Factor, MGF, c-kit Ligand
• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

屬性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in E. coli
• 化驗: >98% (SDS-PAGE and HPLC)
• 比活性: ≥5 x 10e5 U/mg
• 製造商／商標名: Chemicon^®
• 雜質: <0.1 ng/μg Endotoxin (of SCF; 1EU/μg)
• 輸入: sample type hematopoietic stem cell(s)
• NCBI登錄號: NM_000899.3; NM_003994.4
• UniProt登錄號: P21583
• 運輸包裝: dry ice
• 基因資訊: human ... KITLG(4254)

描述

一般說明

Product Source: E.coli

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human SCF is an 18.4 kDa protein containing 165 amino acid residues corresponding to the soluble secreted form of SCF.

應用

For most in vitro applications, SCF exerts its biological activity in the concentration range of 0.2 to 20.0 ng/mL. Responding cells are (partial list): Stem cells, BM precursor cells.

外觀

The sterile filtered solution was lyophilized from 10mM Acetic acid.

儲存和穩定性

The lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be further diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. Reconstituted SCF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.

分析報告

Specific Activity: Chemicon′s human SCF is fully biologically active when compared to standards.The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2 ng/mL

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1