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重要文件

EZHTNFA-150K

Millipore

Human TNF-α ELISA Kit

detects TNF-α in 50 μL serum, plasma or cell culture supernatent

同義詞:

Human TNF-A ELISA, TNF-A ELISA, TNF-Alpha, Tumour Necrosis Factor Alpha ELISA

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.77
暫時無法取得訂價和供貨情況

產品名稱

Human TNF-α Conferma® ELISA Kit, kit sufficient for 96 wells

品質等級

物種活性

human

包裝

kit of 1 × 96 wells

參數

50 μL sample volume (4hr assay)

assay range

linearity: 89-113%
(Serum, 101%)

linearity: 98-110%
(Plasma, 103%)

recovery range: 81-111%
(Plasma, 97%)

recovery range: 85-105%
(Serum,95%)

inter-assay cv: ≤7%
intra-assay cv: ≤3%
sensitivity: 1.65 pg/mL
standard curve range: 1.65-400 pg/mL

技術

ELISA: suitable

輸入

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

應用

research use

檢測方法

colorimetric (450nm/590nm)

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

TNF-α is a core biomarker for the study of inflammation, considered to be a major contributing factor to numerous diseases and pathological processes. The Conferma® ELISA for TNF-α uses highly characterized critical reagents assessed by LC-MS, RPLC-UV-MS and Surface Plasmon Resonance (SPR) to deliver ensure similarity and that the mAb / Standard relationship is maintained . Each assay is further anchored against a recombinant gold standard and a sample library to ensure strong finished kit performance lot to lot. Detailed information can be found in the Protocol & Assay Verification Report.

Research Category: Inflammation

Research Sub-Category:
Alzheimer′s Disease
Aging
Inflammation
Neurodegenerative Diseases
Metabolic Disease
Sepsis Viral Diseases
Cardiovascular Disease

特異性

This assay was tested for cross reactivity with other human cytokines like IL-1a, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17A, CCL3, CCL4, MCP-1, TNFα, GMCSF, VEGF. There is no cross-reactivity detected with any analyte.

應用

For the detection of TNF-α by ELISA in Human Serum & Plasma.

特點和優勢

Capture ELISA assay. Highly Verified. Suitable for 37 samples in Duplicate, with a 6 point Standard Curve, 3 Quality Controls and a Blank.

法律資訊

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

標靶器官

Respiratory Tract

儲存類別代碼

8A - Combustible, corrosive hazardous materials

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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文章

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

瞭解 Conferma® ELISA 開發和製造方法,可提供強大的樣品檢測能力和長期的檢測與批次一致性,讓您對研究充滿信心。

條款

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

本文提供 4 種常用的 ELISA 協定:三明治式 ELISA 協定、磷酸化分析步驟、EIA 分析步驟及細胞式分析步驟。

相關內容

通过适合的免疫检测产品,探索神经免疫和神经炎症细胞因子及神经退行性疾病生物标志物。

Find immunoassays to explore neuroimmunology and neuroinflammation cytokines and neurodegenerative disease biomarkers.

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