CC076

Human Collagen Type IV

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

屬性

• product name: 人IV型胶原蛋白,
• 生物源: human
• 品質等級: 100
• 化驗: 95% (Human collagen type IV, SDS-PAGE)
• 形狀: liquid
• 分子量: 300 kDa
• 製造商／商標名: Chemicon^®
• 濃度: 1 mg/mL
• 技術: cell culture | mammalian: suitable
• 雜質: <5% Human collagen types I-III, V, VI, and non-collagen proteins
• 輸入: sample type epithelial cells; sample type hematopoietic stem cell(s); sample type pancreatic stem cell(s); sample type neural stem cell(s); sample type mesenchymal stem cell(s); sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• 溶解度: water: soluble at 20 °C
• NCBI登錄號: NM_001845.4
• UniProt登錄號: P02462
• 結合特異性: Peptide Source: Laminin; ; Peptide Source: Nidogen
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 基因資訊: human ... COL4A1(1282)

描述

一般說明

Human collagen type IV is a major structural element predominantly found in the basement membrane of the skin. It is a native triple helix and heterotrimeric protein containing two α1-like and one α2-like genes. Type 4 collagen forms a sheet-like extracellular matrix in multicellular tissues that exists around certain cell types like adipocytes, skeletal muscle cells, heart muscle cells, and smooth muscle cells. Human Collagen IV is extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type 4 was purified by using differential salt precipitation. Purity and retention of the native helical structure were controlled by SDS-PAGE and by reaction with anti-collagen type-specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL Product Source: Human placenta, negative for HBsAg and HIV antibodies

應用

Collagen IV from the human placenta is suitable for use as a coating material for cell culture studies and the formation of collagen gels. Human Collagen Type IV has been used: to prepare collagen gel for the invasion assay to provide a supportive matrix for assessing the invasive capacity of monocytes in a cell culture systemas an extracellular matrix (ECM) protein in microarray analysis, to coat nitrocellulose film slidesto coat the microchannels of the P96-O2 plate, and provide a surface for the adhesion and growth of endothelial cells (hMVECs) and renal proximal tubular epithelial cells (hRPTECs) in a co-culture system

生化／生理作用

Human collagen type IV is associated with Alport and Goodpasture′s syndrome, as well as several rheumatological and dermatological diseases including, acquired epidermolysis bullosa.

外觀

液体纯化蛋白。 在100μ L的0.5 M乙酸溶液中，pH 2.5。 无盐，无防腐剂。

儲存和穩定性

可在-20ºC保存长达12个月。 应避免反复冻/融循环。

分析報告

通过形成微纤维的能力证实了天然结构的保留。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable