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F2006

Sigma-Aldrich

Human Fibronectin

from human plasma, powder, suitable for cell culture

同義詞:

纤连蛋白 溶液 来源于人类成纤维细胞

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About This Item

EC號碼:
289-149-2
MDL號碼:
MFCD00131062
分類程式碼代碼:
12352202
eCl@ss:
42020141
NACRES:
NA.75
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產品名稱

人血浆纤连蛋白, lyophilized powder, BioReagent, suitable for cell culture

生物源

human plasma

品質等級

200

無菌

sterile

產品線

BioReagent

化驗

≥85% (SDS-PAGE)

形狀

lyophilized powder

分子量

450 kDa

包裝

pkg of 5 x 5 mg
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

濃度

>1 mg/mL protein

技術

cell culture | mammalian: suitable

表面覆盖率

1‑5 μg/cm2

雜質

HBSAG, none detected
 HCV, none detected
 HIV-1/HIV-2, none detected

溶解度

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI登錄號

NM_212482.4

UniProt登錄號

P02751

結合特異性

Peptide Source: Collagen

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... FN1(2335)

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相關類別

一般說明

纤连蛋白（FN）是一种多功能糖蛋白，相应的基因定位于人染色体2q34-36。该基因有75kb长并含有50个外显子。纤连蛋白结构中包含多个重复肽序列，这些序列在本质上是同源的，并在两个亚基上形成线性排列的功能单元。[1]它存在有两种主要的异构体，一个可溶而另一种不可溶。前者存在与血浆中，而不可溶形式则存在于组织和软骨的细胞外基质（ECM）中。因在不同的区域存在可变剪切，故存在约20种不同的纤连蛋白形式，其中有两个III型外显子的被称为Extra Domains A和B。[2]

應用

来自人血浆的纤连蛋白可用于以下应用：
  • 细胞接种和神经元分化（作为CBSC（脐带血干细胞）重悬的扩增培养基的组分之一，并用于分化培养基）[3]
  • 细胞培养（以纤连蛋白包被培养小室进行HEK293细胞的表达）[4]
  • 体外纤连蛋白（FN）沉淀试验[5]
  • 蛋白特异性[6]
  • 用于细胞迁移研究[7]

生化／生理作用

纤连蛋白（FN）在多种生物学功能中起着必不可少的作用，如血管生成、细胞迁移、细胞-细胞粘附、细胞-基膜粘附、血栓稳定、胚胎发生、分化、伤口愈合以及病原体与哺乳细胞和胞外基质的结合。 [1] 对中国汉族人群的研究表明该基因的突变可能与膝关节骨性关节炎（OA）的易感性存在关联。[2]FN的的剪切体extra domain-B（EDB）是肿瘤血管生成的标志，并随着基质硬度的增加，内皮细胞而产生的这种异构体也会增加。[8]在结直肠癌（CRC）中，EDA与肿瘤转移、血管生成和肿瘤生成存在关联，并可促进上皮-间充质转化。[9]

注意

该重悬溶液应按工作溶液进行分装并储存在-20°C或更低温度下。 不推荐对重悬的纤连蛋白进行涡旋、过度的摇晃或额外的反复冻融。

準備報告

该产品是由pH 7.5的0.05 M Tris盐缓冲液冻干而得。 以1 mg/mL浓度溶于水，在37°C下溶解至少30分钟。 少量未溶解的物质可能会残留，但不会影响性能。 通过免疫电泳评价蛋白的均匀性，检测内毒素，源材料HIV、乙肝、丙肝和梅毒检测均为阴性。 在涂覆培养表面时，纤连蛋白应使用无菌平衡盐溶液进行稀释，并以最小体积进行涂覆。 表面随后应在室温下空气干燥45分钟，并可在2-8°C储存2-4周。

免責聲明

仅供研究使用。本品在法国的科研应用受到监管，包括用于进口和出口活动（法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2）。如《公共卫生法典》所述，购买者（即最终用户）需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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分析證明 (COA)

Lot/Batch Number
0000373118
0000384627
0000362677
SLCQ4732
0000329201

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存取文件庫

Fibronectin: a brief overview of its structure, function, and physiology
Proctor R A, et al.
Reviews of Infectious Diseases, 4, S317-S321 (1987)
Timothy E Quan et al.
Methods in molecular medicine, 135, 423-434 (2007-10-24)
Fibrocytes circulate in the peripheral blood, produce collagen and other matrix proteins, and express cell surface markers indicative of a hematopoetic origin distinguishing them from fibroblasts. Circulating fibrocytes were first identified in 1994 in a model system of wound repair
Kerim B Kaylan et al.
Integrative biology : quantitative biosciences from nano to macro, 8(12), 1221-1231 (2016-11-02)
Carcinoma progression is influenced by interactions between epithelial tumor cells and components of their microenvironment. In particular, cell-extracellular matrix (ECM) interactions are known to drive tumor growth, metastatic potential, and sensitivity or resistance to therapy. Yet the intrinsic complexity of
Marcin Jurga et al.
Biomaterials, 32(13), 3423-3434 (2011-02-18)
Currently, there are no effective therapies to restore lost brain neurons, although rapid progress in stem cell biology and biomaterials development provides new tools for regeneration of central nervous system. Here we describe neurogenic properties of bioactive scaffolds generated by
Chia-Wen Chang et al.
Lab on a chip, 14(19), 3762-3772 (2014-08-07)
This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) hybrid microfluidic device capable of performing cell culture under combinations of chemical and oxygen gradients. The microfluidic device is constructed of two PDMS layers with microfluidic channel patterns separated by a thin PDMS membrane.
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文章

纖維連接素

纖維連接素 (FN) 在細胞外基質纖維組裝和細胞互作中扮演關鍵角色。

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

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條款

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

纖維連接素塗層協議

稀釋纖維連接素用於細胞附著，因細胞類型而異。提供塗佈方案、產品及常見問題。

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定義的培養條件。人類癌症細胞株在 PromoCell 的無血清/無毒素癌症細胞株培養基-XF 中持續生長。在 Sigma.com 探索超過 350 種 PromoCell 產品

Questions

1–9 of 9 Questions  

  1. How do I test the wells/flasks that have been coated with fibronectin?

    1 answer

    1. We test the coated culture surface by culturing cells.  We compare the adherence of the cells to wells that have been coated with media containing BSA (negative) and media containing 10% FBS (positive).  The cell growth should be not less than 50% of the positive control and greater than 50% of the negative control.

      Helpful?

  2. How do I determine how much fibronectin is in solution?

    1 answer

    1. As the product may contain undissolved material, the amount of fibronectin in solution can be determined by UV absorbance.  The extinction coefficient for a 1% solution (10 mg/ml) is 13.5 at 280 nm.

      Helpful?

  3. What is the procedure for coating culture surfaces with fibronectin?

    1 answer

    1. To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume.  2. Allow to air dry for at least 45 minutes at room temperature.  Excess fibronectin may be removed by aspiration, but this is not necessary.

      Helpful?

  4. How do I solubilized Fibronectin?

    1 answer

    1. Dissolve at 1 mg/mL in water. Allow to dissolve for at least 30 minutes at 37 °C. A small amount of undissolved material may remain. This will not affect product performance.

      Helpful?

  5. How do I choose which fibronectin product to use to coat culture surfaces for cell growth?

    1 answer

    1. The species source for the fibronectin used when coating culture surfaces is not important.  It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.

      Helpful?

  6. How long can I store fibronectin coated plates, etc?

    1 answer

    1. Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.

      Helpful?

  7. What is the difference between Fibronection Products F2006 and F1056?

    1 answer

    1. The difference between Product Nos. F2006 and F1056 is in the purity specifications and endotoxin testing.  Product  No. F2006 has a purity specification of ≥90% and is not tested for endotoxin contamination.  Product No. F1056 has a purity specification of ≥95% and is tested for endotoxin contamination with a specification of ≤100 EU/mg.

      Helpful?

  8. How long can I store the fibronectin solution?

    1 answer

    1. The product is good for at least a year at 2-8 °C if kept sterile.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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