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AC112C

Sigma-Aldrich

Sheep Anti-Human IgG Antibody, Cy3 conjugate

from sheep, CY3 conjugate

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702

生物源

sheep

品質等級

共軛

CY3 conjugate

抗體表格

purified antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

物種活性

human

技術

immunocytochemistry: suitable

運輸包裝

wet ice

目標翻譯後修改

unmodified

一般說明

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins, which stage immune responses against pathogenic organisms. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc gamma receptors which interact with sialylated Fc fragments on IgG molecules. The coupling of Cy3 to Anti-Human IgG antibody allows for the visualization of proteins by fluorescent microscopy.

特異性

Based on ELISA analysis using the unconjugated antibody (Cat. No. AC112), this antibody exhibits strongest immunoreactivity towards whole human IgG molecule (IgG>IgM>IgG Fc>IgG Fab>IgA>IgM Fc) with minimal or no cross-reactivity toward IgG from chicken, guinea pig, hamster, mouse, rabbit, and rat species.

免疫原

Purified human IgG.

應用

Research Category
Secondary & Control Antibodies
This Sheep Anti-Human IgG, Cy3 conjugate, Cat. No. AC112C, detects human IgG and is validated for Immunocytochemistry staining of human IgG-producing B-cell hybridoma SA13.
This antibody is also available in unconjugated and Cy5-conjugated forms (Cat. Nos. AC112 and AC112S).

品質

Evaluated by Immunocytochemistry in human B-cell hybridoma SA13.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody immunostained 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human B-cell hybridoma SA13, but not HeLa cells.

標靶描述

~150 kDa observed.

外觀

Ammonium sulfate.
Purified sheep polyclonal antibody Cy3 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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