15-116
ATM/Chk2 Pathway Explorer Antibody MiniPack
Upstate®, from mouse, unconjugated
同義詞:
A-T, mutated, AT mutated, TEL1, telomere maintenance 1, homolog, ataxia telangiectasia mutated, ataxia telangiectasia mutated (includes complementation groups A, C
and D), ataxia telangiectasia mutated protein, human phosphatidylinositol 3-kinas
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About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推薦產品
生物源
mouse
品質等級
共軛
unconjugated
抗體產品種類
primary antibodies
無性繁殖
10H11.E12, monoclonal
7, monoclonal
AM9, monoclonal
物種活性
human (05-513SP,05-649SP, 05-740SP), mouse (05-649SP and 05-740SP), rat
製造商／商標名
Upstate®
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
thin layer chromatography (TLC): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... ATM(472)
mouse ... Atm(11920)
rat ... Atm(300711)
一般說明
Pathway Explorer Antibody MiniPack:
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
ATM:
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
Chk2:
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
* See full size versions for corresponding references.
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
ATM:
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
Chk2:
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
* See full size versions for corresponding references.
特異性
05-513SP: Other species not tested.
05-649SP and 05-740SP: Others not tested.
05-649SP and 05-740SP: Predicted to cross-react with rat based on sequence homology.
免疫原
05-649SP: Mixture of two GST fusion proteins corresponding to residues 1-223 and 217-543 of human Chk2;05-740SP: KLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence has 11/12 identical amino acids in mouse and rat.;05-513SP: Full length human ATM. Clone AM9.
Epitope: 05-740SP:Ser1981
應用
Research Category
Signaling
Signaling
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
This Antibody pack contains Anti-ATM Antibody, Anti-phospho-ATM Antibody (Ser1981), Anti-Chk2 Antibody validated for use in WB, ICC & IP.
包裝
Each vial is 30% the size of the parent catalog number
成分
05-513SP Anti-ATM, clone AM9
05-740SP Anti-phospho-ATM (Ser1981),
clone 10H11.E12
05-649SP Anti-Chk2, clone 7
05-740SP Anti-phospho-ATM (Ser1981),
clone 10H11.E12
05-649SP Anti-Chk2, clone 7
品質
05-649SP: Routinely evaluated by immunoblot on RIPA lysates from Jurkat cells, HeLa, 3T3/A31, or A431 cells.
Western Blot Analysis: 0.1-1 μg/mL of this lot detected Chk2 in RIPA lysates from Jurkat cells. A previous lot detected Chk2 in lysates from HeLa, 3T3/A31, and A431 cells.
05-740SP: Routinely evaluated by immunoblot on in crude lysates from irradiated HeLa cells.
Western Blot Analysis: 0.5 µg/mL of this lot detected phosphorylated ATM in crude lysates from irradiated HeLa cells.
05-513SP: Routinely evaluated by immunoblot on nuclear extract from Raji cells.
Western Blot Analysis:
1:250-1:1000 dilution of this lot detected ATM in a nuclear extract from Raji cells.
Western Blot Analysis: 0.1-1 μg/mL of this lot detected Chk2 in RIPA lysates from Jurkat cells. A previous lot detected Chk2 in lysates from HeLa, 3T3/A31, and A431 cells.
05-740SP: Routinely evaluated by immunoblot on in crude lysates from irradiated HeLa cells.
Western Blot Analysis: 0.5 µg/mL of this lot detected phosphorylated ATM in crude lysates from irradiated HeLa cells.
05-513SP: Routinely evaluated by immunoblot on nuclear extract from Raji cells.
Western Blot Analysis:
1:250-1:1000 dilution of this lot detected ATM in a nuclear extract from Raji cells.
標靶描述
05-649SP: 67 kDa;05-740SP:~370 kDa;05-513SP: 350 kDa
外觀
3 individual tubes containing either Anti-ATM, clone AM9; Anti-phospho-ATM (Ser1981), clone 10H11.E12; or Anti-Chk2, clone 7
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
分析報告
Control
For 05-649SP use Jurkat cell lysate, HeLa cell lysate.
For 05-740SP use Irradiated HeLa cell lysates.
For 05-513SP use Raji cells
For 05-649SP use Jurkat cell lysate, HeLa cell lysate.
For 05-740SP use Irradiated HeLa cell lysates.
For 05-513SP use Raji cells
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Heparin-coated superparamagnetic nanoparticle-mediated adeno-associated virus delivery for enhancing cellular transduction.
Jun-Ho Hwang,Slgirim Lee,Eunmi Kim,Jung-Suk Kim,Chang-Ha Lee,Ik-Sung Ahn,Jae-Hyung Jang
International Journal of Pharmaceutics null
Yongjun Tan et al.
Molecular and cellular biology, 27(3), 1007-1016 (2006-11-15)
The forkhead box M1 (FoxM1) transcription factor regulates expression of cell cycle genes essential for DNA replication and mitosis during organ repair and cancer progression. Here, we demonstrate that FoxM1-deficient (-/-) mouse embryonic fibroblasts and osteosarcoma U2OS cells depleted in
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