The specific isoform is not identified. The SDS PAGE reveals a molecular weight of 47 kDa for this product. However, this molecular weight includes a C-terminal 6His tag (0.8 kDa) and an N-terminal GST tag (approx. 26 kDa). The molecular weight of the remaining recombinant protein is just over 20 kDa. See the link below to review the product datasheet, which includes the sequence:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/610/13-173.pdf
推薦產品
生物源
bovine
品質等級
形狀
liquid
分子量
Mw ~47 kDa
製造商／商標名
Upstate®
濃度
1 mg/mL
技術
activity assay: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
一般說明
Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.
品質
Routinely evaluated by kinase assay
外觀
ProBond Resin chromatography
500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.
儲存和穩定性
Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.
其他說明
Purity information is lot specific, please consult the certificate of analysis
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Vanessa D Stewart et al.
FEBS letters, 596(22), 2952-2973 (2022-09-15)
Myelin-associated glycoprotein (MAG) and Nogo inhibit neurite outgrowth by binding to receptors such as NgR1, PirB and LRP1, and they have also been shown to induce phosphorylation of Smad2, a key intermediate in the transforming growth factor β (TGFβ) signalling
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Which MBP isoform is product 13-173 (SKU)? Can I please have the sequence of it?
1 answer-
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