1.01301

Auramine O (C.I. 41000)

for microscopy

• 同義詞: Auramine O (C.I. 41000), Pyoktamin yellow, 4,4′-Bis-(dimethylamino)-benzophenonimide hydrochloride
• 經驗公式（希爾表示法）: C₁₇H₂₂ClN₃
• CAS號碼: 2465-27-2
• 分子量：: 303.83
• MDL號碼: MFCD00012484
• 分類程式碼代碼: 41116130
• 酶委員會索引編號: 219-567-2

屬性

• 品質等級: 400
• 形狀: solid
• 效力: 1000 mg/kg LD₅₀, oral (Rat)
• pH值: 6-7 (20 °C, 10 g/L in H₂O)
• mp: 265 °C (decomposition)
• 溶解度: 10 g/L
• 體積密度: 430 kg/m³
• εmax: 1100-1300 at 431-433 nm at 0.005 g/L (pH 6.0)
• 應用: diagnostic assay manufacturing; hematology; histology
• 儲存溫度: 2-30°C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• InChI 密鑰: KSCQDDRPFHTIRL-UHFFFAOYSA-N

描述

一般說明

Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol^™ also has a disinfectant effect, with the result that any microorganisms that are present are killed off. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

應用

For microscopy. Staining of mycobacteria according to Hagemann-Hermann, fluorescence staining.
CAS No. 2465-27-2, EC Number 219-567-2.

分析報告

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (buffer pH 6.0)431 - 433 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300TLC-Testpasses testLoss on drying (110 °C)≤ 5 %Suitability for microscopypasses test

法律資訊

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS07,GHS08,GHS09
• 訊號詞: Warning
• 危險聲明: H302 - H319 - H351 - H411
• 防範說明: P201 - P202 - P273 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• 危險分類: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3