1.00327

Hydrogen chloride solution

in ethanol, for microscopy

• 同義詞: 氯化氢乙醇溶液
• 線性公式: HCl · CH₃CH₂OH
• CAS號碼: 7647-01-0
• 分子量：: 36.46
• Beilstein: 1098214
• MDL號碼: MFCD00011324
• 分類程式碼代碼: 41116130
• NACRES: NA.21

屬性

• 產品名稱: 氯化氢 – 乙醇 溶液, in ethanol, for microscopy
• 品質等級: 400
• 形狀: liquid
• IVD: for in vitro diagnostic use
• 技術: microbe id | staining: suitable
• pH值: 1.0 (20 °C in H₂O)
• 轉變溫度: flash point 21 °C
• 密度: 0.90 g/cm³ at 20 °C
• 應用: clinical testing; diagnostic assay manufacturing; hematology; histology
• 儲存溫度: 2-30°C
• SMILES 字串: Cl
• InChI: 1S/ClH/h1H
• InChI 密鑰: VEXZGXHMUGYJMC-UHFFFAOYSA-N

描述

一般說明

Hydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

應用

For microscopy. Ziehl-Neelsen staining

法律資訊

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS02,GHS05
• 訊號詞: Danger
• 危險聲明: H225 - H290 - H319
• 防範說明: P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338
• 危險分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: 69.8 °F
• 閃點（°C）: 21 °C