03-209

SET7/9 human recombinant - Methyltransferase enzyme

Human recombinant SET7/9 expressed in E. Coli.

• 分類程式碼代碼: 12352200
• NACRES: NA.54

屬性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in E. coli
• 化驗: >95% (SDS-PAGE)
• 分子量: ~41 kDa
• 包裝: pkg of 100 μg
• 製造商／商標名: Upstate^®
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: 1 mg/mL
• 技術: enzyme immunoassay: suitable
• UniProt登錄號: Q8WTS6

描述

一般說明

The methylation of histones plays a pivotal role in the regulation of chromatin structure and gene expression. Histone methylation can occur on Arg or Lys residues, with an exquisite site selectivity for Lys methylation at specific positions in the N-termini of histones H3 and H4. SET7/9, a histone methyltransferase (HMTase), which transfers methyl groups to Lys4 of Histone H3, forms a complex with S-adenosyl-L-methionine. This complex contains an active site consisting of a binding pocket where an AdoMet molecule, in an unusual conformation, binds a narrow substrate-specific channel that only unmethylated lysine residues can access, and a catalytic tyrosine residue.

特異性

<1.0EU/µg protein (LAL test)

應用

Human recombinant SET7/9 expressed in E. Coli.

外觀

Format: Purified

In liquid containing 50 mM Tris-HCl, pH 7.5, containing 0.2 M sodium chloride, 5 mM DTT and 20% glycerol. Frozen solution.

儲存和穩定性

Stable for 12 months at -20°C from date of receipt. Avoid multiple freeze/thaw cycles.

分析報告

Methyltransferase assays

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1