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900622

Sigma-Aldrich

明胶甲基丙烯酰基

gel strength 300 g Bloom, degree of substitution 60%

同義詞:

GelMa, 明胶甲基丙烯酰胺, 明胶甲基丙烯酸酯

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About This Item

線性公式:
(C40H59N11O13)n
分類程式碼代碼:
12352202
NACRES:
NA.23
暫時無法取得訂價和供貨情況

品質等級

100

形狀

powder

儲存溫度

2-8°C

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相關類別

應用

Gelatin methacryloyl can be used to form cross-linked hydrogels for tissue engineering[1] and 3D printing[2][3][4]. It has been used for endothelial cell morphogenesis[5], cardiomyocytes[6], epidermal tissue[7], injectable tissue constructs[8], bone differentiation[9], and cartilage regeneration[10]. Gelatin methacryloyl has been explored in drug delivery applications in the form of microspheres[11] and hydrogels[12].

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
MKCT0119
MKCT0116
MKCT0113
MKCS2673
MKCQ6360

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Xin Zhao et al.
Advanced healthcare materials, 5(1), 108-118 (2015-04-17)
Natural hydrogels are promising scaffolds to engineer epidermis. Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based, which mimic the natural dermal extracellular matrix but often suffer from insufficient and uncontrollable mechanical and degradation properties. In
Kelly M C Tsang et al.
Advanced functional materials, 25(6), 977-986 (2015-09-04)
Hydrogels are often employed as temporary platforms for cell proliferation and tissue organization in vitro. Researchers have incorporated photodegradable moieties into synthetic polymeric hydrogels as a means of achieving spatiotemporal control over material properties. In this study protein-based photodegradable hydrogels
Kristel W M Boere et al.
Acta biomaterialia, 10(6), 2602-2611 (2014-03-05)
Hydrogels can provide a suitable environment for tissue formation by embedded cells, which makes them suitable for applications in regenerative medicine. However, hydrogels possess only limited mechanical strength, and must therefore be reinforced for applications in load-bearing conditions. In most
Jason W Nichol et al.
Biomaterials, 31(21), 5536-5544 (2010-04-27)
The cellular microenvironment plays an integral role in improving the function of microengineered tissues. Control of the microarchitecture in engineered tissues can be achieved through photopatterning of cell-laden hydrogels. However, despite high pattern fidelity of photopolymerizable hydrogels, many such materials
Anh H Nguyen et al.
Acta biomaterialia, 13, 101-110 (2014-12-03)
Gelatin has been commonly used as a delivery vehicle for various biomolecules for tissue engineering and regenerative medicine applications due to its simple fabrication methods, inherent electrostatic binding properties, and proteolytic degradability. Compared to traditional chemical cross-linking methods, such as
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相關內容

組織工程

組織工程透過模擬人體的微環境，利用支架、活細胞和生物活性分子製造組織培養物，以修復或取代受損組織。

組織工程

組織工程透過模擬人體的微環境，利用支架、活細胞和生物活性分子製造組織培養物，以修復或取代受損組織。

Tissue Engineering

Tissue engineering fabricates tissues cultures from scaffolds, living cells, and biologically active molecules by simulating the microenvironment of the body to repair or replace damaged tissue.

Questions

1–3 of 3 Questions  

  1. Is this product sterile or not, how to sterilize it?

    1 answer

    1. This product is not sterilized. Sterilization can be achieved by passing a solution through a 0.2 μm PES syringe filter.

      Loessner, D., Meinert, C., Kaemmerer, E. et al. Functionalization, preparation and use of cell-laden gelatin methacryloyl–based hydrogels as modular tissue culture platforms. Nat Protoc 11, 727–746 (2016). https://doi.org/10.1038/nprot.2016.037

      Helpful?

  2. Can you provide guidance on reconstituting the GelMA purchased from Sigma? There is no protocol available online.

    1 answer

    1. There is no protocol available for reconstituting or testing the solubility of the product 900622.
      Here is some information on solubility:
      1.Mix LAP in 3 ml of PBS or cell media at 60°C under stirring to create a 0.5% w/v LAP concentration.
      2.Mix until all LAP is dissolved. Add GelMA to the solution to create a 10% w/v concentration.
      3.Mix at 60°C under stirring until all GelMA is dissolved.

      Helpful?

  3. Sind in dem Produkt Antioxidantien/Inhibitoren wie etwa Topanol A enthalten oder handelt es sich um einen Reinstoff?

    1 answer

    1. The composition of this product is considered proprietary.

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