809705

5-DBCO-PEG₄-dUTP, 10mM Aqueous Solution

• 同義詞: 5-DBCO-PEG₄-dUTP
• 經驗公式（希爾表示法）: C₄₂H₅₄N₅O₂₁P₃
• 分子量：: 1057.82
• 分類程式碼代碼: 12352200
• PubChem物質ID: 329769180
• NACRES: NA.22

屬性

• 化驗: >95% (HPLC)
• 品質等級: 200
• 形狀: solution
• 反應適用性: reaction type: click chemistry
• 溶解度: Tris buffer: 100 mM (pH 7.5)
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• SMILES 字串: O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O

描述

應用

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2