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重要文件

318426

Sigma-Aldrich

碘化3,3′-二己基氧羰花青

98%

同義詞:

3-己基-2-[3-(3-己基-2 (3 H ) 苯并恶唑亚甲基)-1-丙烯基] 苯并恶唑碘化物

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About This Item

經驗公式(希爾表示法):
C29H37IN2O2
CAS號碼:
分子量::
572.52
Beilstein:
9312562
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
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品質等級

化驗

98%

形狀

powder or chunks

技術

titration: suitable

mp

219-221 °C (lit.)

溶解度

chloroform: 25 mg/mL, clear to slightly hazy, orange

λmax

485 nm

&epsilon ;(消光係數)

≥120000 at 483-488 nm in methanol at 0.01 g/L

螢光

λex 485 nm; λem 501 nm in methanol

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

[I-].CCCCCCN1\C(Oc2ccccc12)=C\C=C\c3oc4ccccc4[n+]3CCCCCC

InChI

1S/C29H37N2O2.HI/c1-3-5-7-13-22-30-24-16-9-11-18-26(24)32-28(30)20-15-21-29-31(23-14-8-6-4-2)25-17-10-12-19-27(25)33-29;/h9-12,15-21H,3-8,13-14,22-23H2,1-2H3;1H/q+1;/p-1

InChI 密鑰

XVLXYDXJEKLXHN-UHFFFAOYSA-M

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一般說明

碘代3,3′-二己氧杂羰花青(DiOC6)是用于离体活体染色的亲脂性荧光色素。[1][2]这种阳离子型染料可用于动物和植物细胞的线粒体[3]和内质网染色。[1][2]还可用于植物根和苔类内生子囊菌的快速、一致和高度选择性的荧光染色。[2]DiOC6还可用于检测细胞凋亡早期发生的不同线粒体变化。[3]

應用

碘代3,3′-二己氧杂羰花青已用于:
  • 植物根和苔类内生子囊菌的选择性染色[2]
  • 可视化血栓生成和血小板聚集[3]
  • 可视化氯吡脲(FCF)诱导的线粒体形态变化[4]

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

Microfluidic cell sorting: Towards improved biocompatibility of extracorporeal lung assist devices
Bleilevens C, et al.
Scientific reports, 8(1), 8031-8031 (2018)
Examination of living and fixed gametes and early embryos stained with supravital fluorochromes (Hoechst 33342 and 3, 3?-dihexyloxacarbocyanine iodide)
Luttmer SJ and Longo FJ
Gamete Research, 15(3), 267-283 (1986)
Xueying Zhuang et al.
Mycological research, 113(Pt 4), 417-431 (2008-12-31)
Growth and organelle morphology in the wood rotting basidiomycete fungus Phanerochaete velutina were examined in Petri dishes, on agar-coated slides, and in submerged cultures, using DIC, fluorescence and four-dimensional (4-D; x,y,z,t) confocal microscopy, with several fluorescent probes. Phanerochaete is ideal
Yanyan Zheng et al.
European journal of medicinal chemistry, 125, 902-913 (2016-10-22)
Emodin, a natural anthraquinone derivative isolated from Rheum palmatum L., has been demonstrated to exhibit good anti-cancer effect. In this study, a series of novel quaternary ammonium salts of emodin, anthraquinone and anthrone were synthesized and their anticancer activities were
Ashlesha A Kadam et al.
Biochimica et biophysica acta, 1861(1 Pt A), 2942-2955 (2016-10-23)
Apoptosis Inducing Factor (AIF), a phylogenetically conserved mitochondrial inter-membrane space flavoprotein has an important role in caspase independent cell death. Nevertheless, AIF is also essential for cell survival. It is required for mitochondrial organization and energy metabolism. Upon apoptotic stimulation

文章

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。

Questions

  1. 本製品をクロロホルムで溶解するときの推奨プロトコルはございますでしょうか。クロロホルムは原液でしょうか。 他社の DiOC6 の説明では溶媒として DMSO も挙げられるようですが、本製品の DMSO への溶解性はいかがでしょうか。

    1 answer
    1. No formal protocol exists for dissolving this product in chloroform. The Specification Sheet and the Certificate of Analysis indicate that solubility is tested at 25 mg/mL in chloroform during quality control testing. It is suggested to dissolve up to 25 mg/mL in chloroform and mix well until the solid is dissolved.
      The chemical may be soluble in other solvents. No attempt is made during quality control to test in a range of solvents. It is certainly possible that other companies may test in DMSO or other solvents. If other companies claim DIOC6 is soluble in a particular solvent, product 318426 should also be soluble. Since the only solvent used for solubility testing is chloroform, there is no guarantee product 318426 will meet the solubility claims from other companies.

      Conn's Biological Stains, 10th Edition advises that 3,3?-Dihexyloxacarbocyanine iodide is also soluble in DMSO and methanol. No exact values are provided for the solubility of DiOC6 in either DMSO or methanol. No solubility data is available from PubChem, The Merck Index or Martindale The Extra Pharmacopeia.

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