216720

4′,5′-二溴荧光素

Dye content 95 %

• 同義詞: 溶剂红 72
• 經驗公式（希爾表示法）: C₂₀H₁₀Br₂O₅
• CAS號碼: 596-03-2
• 分子量：: 490.10
• 顏色索引編號: 45370:1
• Beilstein: 57189
• EC號碼: 209-876-0
• MDL號碼: MFCD00005042
• 分類程式碼代碼: 12171500
• PubChem物質ID: 24852976
• NACRES: NA.47

屬性

• 化驗: >95% (TLC)
• 形狀: powder
• 成份: Dye content, 95%
• mp: 270-273 °C (lit.)
• λmax: 450 nm
• &epsilon ；(消光係數): ≥5000 at 445-451 nm; ≥50000 at 227-233 nm
• 應用: diagnostic assay manufacturing; hematology; histology
• 儲存溫度: room temp
• SMILES 字串: Oc1ccc2c(Oc3c(Br)c(O)ccc3C24OC(=O)c5ccccc45)c1Br
• InChI: 1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
• InChI 密鑰: ZDTNHRWWURISAA-UHFFFAOYSA-N

描述

一般說明

4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.

應用

4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.

安全資訊

• 象形圖: GHS06
• 訊號詞: Danger
• 危險聲明: H301
• 防範說明: P301 + P310
• 危險分類: Acute Tox. 3 Oral
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges