The kit is optimized for handling sample weights of 0.1 to 1 gram and produces a colorimetric result proportional to glucose concentration. However, the detection limit has not been determined for this assay kit.
SA20
Starch (HK) Assay Kit
sufficient for 20 assays
Synonym(s):
Starch Quantitation Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12164500
Technique(s):
photometry: suitable
Usage:
sufficient for 20 assays
Storage temp.:
2-8°C
Pricing and availability is not currently available.
usage
sufficient for 20 assays
Quality Segment
analyte chemical class(es)
sugars
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Application
This kit is for the quantitative, enzymatic determination of native starch in food and other materials using the amyloglucosidase-hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay. The hydrolysis of starch to glucose is catalyzed by amyloglucosidase. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The increase in absorbance at 340 nm is directly proportional to the glucose concentration.
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This Item | |||
|---|---|---|---|
| technique(s) photometry: suitable | technique(s) photometry: suitable | technique(s) photometry: suitable | technique(s) photometry: suitable |
| usage sufficient for 20 assays | usage sufficient for 20 assays | usage sufficient for 20 assays | usage sufficient for ~100 assays |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
| analyte chemical class(es) sugars | analyte chemical class(es) sugars (glucose) | analyte chemical class(es) sugars (fructose) | analyte chemical class(es) - |
Kit Components Only
Product No.
Description
- glucose assay reagent 20 mL
- starch assay reagent 1 mL/vial
- starch assay standard 5 g
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
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SA20スターチ（HK）アッセイキットの感度と検出限度を教えて下さい。 よろしくお願いいたします。
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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