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S6782

Superdex®

Superdex®200 Prep Grade

Synonym(s):

Size exclusion media, Superdex column

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
MFCD00166816
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Quality Segment

200

form

suspension (20% aqueous ethanol)

technique(s)

protein array: suitable

matrix

cross-linked agarose

particle size

24-44 μm

pore size

1-100 kDa MW range (dextrans), 10-600 kDa MW range (proteins)

operating pH range

1-14(short term), 3-12(long term)

storage temp.

2-8°C

General description

Combines the high selectivity of a Sephadex matrix with the chemical and physical stability of crosslinked agarose.

Application

Optimized for high resolution preparative separations by size exclusion chromatography. Recommended fractionation ranges are listed for globular proteins and dextrans.
Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.

Legal Information

Superdex is a registered trademark of Cytiva

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This Item
S6657CL4B200S300HR
Superdex® Superdex®200 Prep Grade

Sigma-Aldrich

S6782

Superdex®

Superdex® Superdex®75 Prep Grade

Sigma-Aldrich

S6657

Superdex®

Sepharose™ CL-4B Cross-linked

Supelco

CL4B200

Sepharose™ CL-4B

Sephacryl® 300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)

Sigma-Aldrich

S300HR

Sephacryl®

particle size

24-44 μm

particle size

24-44 μm

particle size

-

particle size

25-75 μm (wet)

matrix

cross-linked agarose

matrix

cross-linked agarose

matrix

Agarose

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

pore size

1-100 kDa MW range (dextrans), 10-600 kDa MW range (proteins)

pore size

3-70 kDa MW range (proteins), 500-30,000 Da MW range (dextrans)

pore size

30,000-5,000,000 fractionation range (Dextrans), 60,000-20,000,000 fractionation range (Globular proteins)

pore size

1-400 kDa MW range (dextrans), 10-1500 kDa MW range (globular proteins)

technique(s)

protein array: suitable

technique(s)

protein array: suitable

technique(s)

LPLC: suitable

technique(s)

-

form

suspension (20% aqueous ethanol)

form

suspension (20% aqueous ethanol)

form

beads

form

suspension (20% aqueous ethanol)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

104.0 °F

flash_point_c

40 °C

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