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Medium 199

Name: Medium 199
Brand: SIGMA

HEPES modification, with Earle′s salts, and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Cell culture medium, M199 Medium, TCM 199

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Medium 199, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO₃: yes
Earle’s salts (5% CO₂): yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Preparation Note

Supplement with 0.1 g/L L-glutamine.

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1 of 1

This Item	M0650	M5017	M9163
Sigma-Aldrich M7528 Medium 199	Sigma-Aldrich M0650 Medium 199	Sigma-Aldrich M5017 Medium 199	Sigma-Aldrich M9163 Medium 199
sterility sterile-filtered	sterility sterile-filtered	sterility -	sterility sterile-filtered
form liquid	form liquid	form powder	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components L-glutamine: no sodium pyruvate: no HEPES: 25 mM phenol red: yes NaHCO₃: yes Earle’s salts (5% CO₂): yes	components NaHCO₃: no phenol red: yes sodium pyruvate: no HEPES: no Earle’s salts (5% CO₂): yes L-glutamine: no	components L-glutamine: yes phenol red: yes NaHCO₃: no sodium pyruvate: no Earle’s salts (5% CO₂): yes HEPES: no	components phenol red: yes HEPES: no sodium pyruvate: no Hanks’ salts (2% CO₂): yes L-glutamine: no NaHCO₃: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

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