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MOPS-EDTA-Sodium Acetate (MESA)

Name: MOPS-EDTA-Sodium Acetate (MESA)
Brand: SIGMA

powder for RNA electrophoresis buffers

Synonym(s):

MESA Buffer

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

Pricing and availability is not currently available.

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Properties

grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H₂O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

Description

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Preparation Note

1x MESA buffer contains:

40 mM MOPS
10 mM sodium acetate
1 mM EDTA (pH 8.3)

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

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This Item	T8280	T7527	PPB008
Sigma-Aldrich M5755 MOPS-EDTA-Sodium Acetate (MESA)	Sigma-Aldrich T8280 Tris Acetate-EDTA buffer	Sigma-Aldrich T7527 Tris-Borate-EDTA buffer	Sigma-Aldrich PPB008 Tris Acetate-EDTA buffer
technique(s) electrophoresis: suitable	technique(s) -	technique(s) -	technique(s) -
grade Molecular Biology	grade Molecular Biology	grade Molecular Biology	grade -
product line BioReagent	product line BioReagent	product line BioReagent	product line pHast Pack^™
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level -
form powder	form powder blend	form powder blend	form powder
pH 6.8-7.2(reconstituted per directions)	pH 8.1-8.5	pH 8.1-8.5 (5 ×)	pH 8.3

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000532868

0000439909

0000383887

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

โปรโตคอล Northern & Southern Blot

เรียนรู้พื้นฐานการแบ่งส่วนภาคเหนือและใต้ด้วยโปรโตคอลและการใช้งานสำหรับการถ่ายโอนโมเลกุลไปยังเมมเบรนสนับสนุน

สูตรและวิดีโอของเทและ TBE Running Buffers

Tae และ TBE ใช้เป็นบัฟเฟอร์สำหรับอิเล็กโตรโฟเรซิสของกรดนิวคลีอิกแต่มีความแตกต่างที่สำคัญบางอย่าง ทบทวนสูตรและวิดีโอของเราเพื่อให้แอปพลิเคชันของคุณมีโอกาสประสบความสำเร็จสูงสุด

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