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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Earle’s salts (5% CO₂): yes
NaHCO₃: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids.[1] It is widely used as a media formulation for many cell culture studies.[2] It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.[1]

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells[3], and endometrial adenocarcinoma Ishikawa cells.[4]

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

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This Item	M7278	M3024	M4526
Sigma-Aldrich M0643 Minimum Essential Medium Eagle	Sigma-Aldrich M7278 Minimum Essential Medium Eagle	Sigma-Aldrich M3024 Minimum Essential Medium Eagle	Sigma-Aldrich M4526 Minimum Essential Medium Eagle
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form liquid	form powder	form liquid
components HEPES: no Earle’s salts (5% CO₂): yes NaHCO₃: no phenol red: yes L-glutamine: yes sodium pyruvate: no	components L-glutamine: no phenol red: yes HEPES: 25 mM sodium pyruvate: no Earle’s salts (5% CO₂): yes NaHCO₃: yes	components Earle’s salts (5% CO₂): yes L-glutamine: no HEPES: no NaHCO₃: no sodium pyruvate: no phenol red: no	components Earle’s salts (5% CO₂): yes sodium pyruvate: yes glucose: yes L-glutamine: no HEPES: no Hanks’ salts (2% CO₂): no phenol red: yes NaHCO₃: yes stable glutamine: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
Quality Level 500	Quality Level 400	Quality Level 500	Quality Level 400

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000500472

0000500470

25D291

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Protocols

Powdered Media Preparation Instructions

สารผสมผงและเกลือเป็นสารดูดความชื้นอย่างมากและควรได้รับการปกป้องจากความชื้นในบรรยากาศ

Articles

MEM (อีเกิ้ลขนาดกลางที่จำเป็นขั้นต่ำ)

มีสูตร MEM ให้เลือกมากมาย Minimum Essential Medium (MEM) ที่พัฒนาโดย Harry Eagle เป็นหนึ่งในสื่อวัฒนธรรมเซลล์สังเคราะห์ที่ใช้กันอย่างแพร่หลายมากที่สุด

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Water for Mammalian Cell Culture

High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.

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