F8180

FMS (539-end), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): C-FMS, CD115, CSF1R, CSFR, FIM2
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 18-24 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 18-24 nmol/min·mg
• mol wt: ~76 kDa
• UniProt accession no.: P07333
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CSF1R(1436)

Description

General description

FMS, also called CSF1R colony stimulating factor 1 receptor, acts as a receptor for CSF1, and they both as key regulators of monocyte and macrophage functioning.[1] It is a member of the platelet-derived growth factor (PDGF) family of proteins, and is a type III RTK (receptor tyrosine kinase). It is composed of five immunoglobulin (Ig)-like domains, a transmembrane region, a juxtamembrane domain, and kinase insert domain (KID) intersecting the kinase domain.[2]

Biochem/physiol Actions

FMS also called CSF1R colony stimulating factor 1 receptor, and its ligand CSF1 are linked to poor prognosis in patients with solid tumors, and in patients with female reproductive cancers. In breast cancers with decreased claudin levels, this protein regulates the switch between proliferative and invasive state of the tumor, acting downstream of TGFβ (tumor growth factor β).[3] Haploinsufficiency in this receptor might lead to aberration of microglial function, which might be a contributor to the pathogenesis of hereditary diffuse leukoencephalopathy with spheroids (HDLS).[4][2]

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)