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Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

Name: Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Sheep Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162642

Conjugate:

FITC conjugate

Clone:

polyclonal

Application:

IF (d)

Citations:

Pricing and availability is not currently available.

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Properties

biological source

sheep

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:32

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule)-FITC antibody is specific for all mouse IgG subclasses. Affinity isolated antigen specific antibody is obtained from sheep anti-mouse IgG antiserum by immunospecific purification. The antibody preparation is then conjugated to Sigma Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a working antibody dilution of 1:32. For immunofluorescence of human ovarian adenocarcinoma cells stained with anti-integrin primary antibodies a working dilution of 1:50 was used. Immunolocalization of human spermatids was done using antibody dilution of 1:100. This antibody was also used for immunofluorescent tagging of leaf cells of Arabidopsis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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1 of 1

This Item	F0257	F2012	F9137
Sigma-Aldrich F6257 Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep	Sigma-Aldrich F0257 Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat	Sigma-Aldrich F2012 Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat	$Anti-Mouse IgG (whole molecule)−FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich F9137 Anti-Mouse IgG (whole molecule)−FITC antibody produced in rabbit
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate
technique(s) direct immunofluorescence: 1:32	technique(s) direct immunofluorescence: 1:32	technique(s) direct immunofluorescence: 1:64 (minimum)	technique(s) direct immunofluorescence: 1:100, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
biological source sheep	biological source goat	biological source goat	biological source rabbit
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000385099

0000261480

0000261479

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PloS one, 8(1), e53532-e53532 (2013-01-24)

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