F5900
Monoclonal Anti-Interleukin-2−Carboxyfluorescein antibody produced in mouse
clone 5334, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-Interleukin-2, Monoclonal Anti-IL-2
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About This Item
NACRES:
NA.41
UNSPSC Code:
51111800
Conjugate:
carboxyfluorescein conjugate
Clone:
5334, monoclonal
Application:
FACS
Citations:
3
Pricing and availability is not currently available.
biological source
mouse
Quality Level
conjugate
carboxyfluorescein conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5334, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
flow cytometry: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... IL2(3558)
General description
Interleukin-2 (IL-2) also refers as T Cell Growth Factor is a cytokine produced by certain subsets of T lymphocytes. It plays various functions like inducing interferon γ and B cell growth factor secretion as well as affecting the activation and proliferation of NK cells. It also has a vital role in modulating the expression of the IL-2 receptor and can facilitate B cell growth and differentiation. Monoclonal anti-Interleukin-2−Carboxyfluorescein antibody reacts specifically with human IL-2.
Immunogen
recombinant human IL-2
Application
Monoclonal anti-Interleukin-2−Carboxyfluorescein antibody can be used in flow cytometry assays for identifying intracytoplasmic cytokines and for enumerating cytokine producing cells present in a mixed population.
Biochem/physiol Actions
The conjugates will identify and quantitate cells containing intracellular forms of human IL-2 by flow cytometry.
Physical form
Solution in phosphate buffered saline containing 0.1% sodium azide.
Preparation Note
Purified antibody is conjugated to carboxyfluorescein.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Instructions
D A Morgan et al.
Science (New York, N.Y.), 193(4257), 1007-1008 (1976-09-10)
Selective growth of T lymphocytes occurred when unfractionated normal human bone marrow cells were cultured with conditioned medium obtained from phytohemagglutinin-stimulated normal human lymphocytes (Ly-CM). Cultures of up to 90 percent T cells have been maintained for more than 9
T A Waldmann et al.
The Journal of experimental medicine, 160(5), 1450-1466 (1984-11-01)
Using anti-Tac, a monoclonal anti-interleukin 2 (IL-2) receptor antibody, we have explored the possibility that certain activated B cells display receptors for IL-2. Resting normal B cells and unselected B cell lines established from normal individuals were Tac antigen negative.
K A Smith et al.
Proceedings of the National Academy of Sciences of the United States of America, 82(3), 864-868 (1985-02-01)
The cell surface density of high-affinity membrane receptors for the T-lymphocytotrophic hormone interleukin 2 (IL-2) determines the rate of T-cell-cycle progression. Since 10-fold greater numbers of IL-2 receptor molecules were found by using a radiolabeled monoclonal antibody reactive with IL-2