D9692
Plant Protoplast Digest/Wash Solution
Rapid isolation of viable protoplasts from plant tissue
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About This Item
NACRES:
NA.56
UNSPSC Code:
41105500
Pricing and availability is not currently available.
General description
The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by a rigid, semi-permeable cell wall composed primarily of three classes of polysaccharides: cellulose, hemicellulose, and pectin. The Plant Protoplast Digest/Wash Solution can be used for the digestion of the cell wall after the addition of enzymes that hydrolyze these polysaccharides, such as cellulase, pectinase, or pectolyase. The Plant Protoplast Digest/Wash Solution can be subsequently used to wash away any remaining hydrolytic enzymes after digestion is complete.
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression,[1] viral transfection assays,[2] somatic hybridization,[3] electrophysiological studies, and morphological studies.[4]
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression,[1] viral transfection assays,[2] somatic hybridization,[3] electrophysiological studies, and morphological studies.[4]
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This Item | |||
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| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 15-25°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form - | form - |
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Anna Kärkönen et al.
Methods in molecular biology (Clifton, N.J.), 715, 1-20 (2011-01-12)
Plant tissue cultures are an efficient system to study cell wall biosynthesis in living cells in vivo. Tissue cultures also provide cells and culture medium where enzymes and cell wall polymers can easily be separated for further studies. Tissue cultures
Shiu-Cheung Lung et al.
Plant cell reports, 30(4), 473-484 (2010-11-26)
Although transient gene expression using reporters such as green fluorescent protein is a versatile tool for examining gene functions and intracellular protein trafficking, the establishment of a highly efficient gene manipulation method remains a challenge in many plant species. A
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White, P. J. et al
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