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C8772

Cholera filtrate

lyophilized powder, bacterial sialidase receptor-destroying enzyme (RDE)

Synonym(s):

Cholera Filter

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
Form:
lyophilized powder
Quality level:
300
Pricing and availability is not currently available.
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Product Name

Cholera filtrate, lyophilized powder

form

lyophilized powder

Quality Level

300

storage temp.

2-8°C

General description

Cholera filtrate promotes the activation of adenylate cyclase. It does not affect the activity of cyclic adenosine monophosphate (cAMP) phosphodiesterase.[1]

Application

Cholerafiltrate has been used as a receptor destroying enzyme (RDE): inhemagglutination inhibition assay of serum samples[2], in microneutralizationassay of mice serum samples, in hemagglutination inhibition assay to removenon-specific inhibitors from the cell culture supernatant samples[3]

Biochem/physiol Actions

Cholera filtrate is a bacterial sialidase receptor-destroying enzyme (RDE) and may be used as crude neuraminidase. It may also be used in serological testing for influenza.

Preparation Note

After reconstituting with 5ml sterile water, it will contain 0.01% MIT as preservative.

Other Notes

See a separate listing of neuraminidase for preparations showing higher activity.

Disclaimer

For research use only. Not for use in diagnostic procedures.

signalword

Danger

flash_point_f

Not applicable

flash_point_c

Not applicable

supp_hazards

EUH071

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1A - Skin Sens. 1

Storage Class

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
0000490167
0000490167
0000403428
0000403428
0000149683

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Applying valency-based immuno-selection to generate broadly cross-reactive antibodies against influenza hemagglutinins
Daniela M, et al.
Nature Communications (2024)
WHO Tech Rep. Serv.
WHO Expert Committee on Influenza, 64 (1953)
A V Kornev et al.
Biulleten' eksperimental'noi biologii i meditsiny, 88(10), 414-416 (1979-10-01)
An in vitro model using homogenates of the rat intestine and liver for studying the V. Cholerae culture filtrate effect on the adenylate cyclase system is proposed. Optimal conditions for the adenylate cyclase functioning have been investigated for this model.
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Questions

1–2 of 2 Questions  

  1. Since it is lyophilized powder, can reagent C8772 be received at room temperature?

    1 answer

    1. This Cholera filtrate powder is shipped ambient.

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  2. The reconstituted solution should be frozen at -20 °C in aliquots ?

    1 answer

    1. The stability of this material in solution has not been evaluated. However, this is a crude source of neuraminidase. The purified neuraminidase product N7885 is an aqueous solution at pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2. This product has an expected shelf life of at least 2 years when stored refrigerated at 2-8°C. Similar stability may be demonstrated by the Cholera Filtrate.

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