C7594

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

• Synonym(s): blocking buffer
• UNSPSC Code: 12161700
• EC Number: 232-555-1
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Segment: 200
• form: powder blend
• technique(s): Southern blotting: suitable
• pH: 6.8-7.2(reconstituted per directions)
• solubility: H₂O: soluble, faintly yellow (dissolves in 5-10 minutes.)
• storage temp.: room temp

Description

General description

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Application

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Features and Benefits

• Better blocking performance than dried milk or BSA
• Compatible with neutral and positively-charged membranes
• Compatible with chemiluminescent, colorimetric , and DIG detection assays

Preparation Note

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

Safety Information

• wgk: WGK 1
• Storage Class: 11 - Combustible Solids