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Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

Name: Choloylglycine Hydrolase from <I>Clostridium perfringens (C. welchii)</I>
Brand: SIGMA

lyophilized powder, ≥100 units/mg protein

Synonym(s):

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

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About This Item

CAS Number:

37289-07-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.5.1.24 (BRENDA, IUBMB)

MDL number:

MFCD00130789

Specific activity:

≥100 units/mg protein

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Properties

form

lyophilized powder

Quality Level

200

specific activity

≥100 units/mg protein

composition

Protein, ≥50% biuret

shipped in

wet ice

storage temp.

−20°C

Description

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.[1][2]

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.[3]

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Other Notes

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

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1 of 1

This Item	G2878	700212P	700189P
Sigma-Aldrich C4018 Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)	Sigma-Aldrich G2878 Glycocholic acid hydrate	Avanti 700212P Cholic acid	Avanti 700189P 3α,6β,7α,12α-tetrahydroxy bile acid (THBA)
specific activity ≥100 units/mg protein	specific activity -	specific activity -	specific activity -
form lyophilized powder	form powder	form powder	form powder
storage temp. −20°C	storage temp. room temp	storage temp. −20°C	storage temp. −20°C
shipped in wet ice	shipped in ambient	shipped in dry ice	shipped in dry ice
Quality Level 200	Quality Level 200	Quality Level -	Quality Level -
composition Protein, ≥50% biuret	composition -	composition -	composition -

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Effects of Quercetin and Rutin on Serum and Hepatic Lipid Concentrations, Fecal Steroid Excretion and Serum Antioxidant Properties

Nakamura Y, et al.

Journal of Health Science, 46(4), 229-240 (2000)

Bile salt hydrolase activity in probiotics.

Máire Begley et al.

Applied and environmental microbiology, 72(3), 1729-1738 (2006-03-07)

Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product.

Maksim Rossocha et al.

Biochemistry, 44(15), 5739-5748 (2005-04-13)

Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction