The product is packaged in bottomless glass bottles with an inserted fused cone. The micro-vials contain the product within an inner cone, while the bottomless outer glass cylinder, serves as a stand. This design is intentional and not a defect.
B4875
Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride
trypsin substrate, chromogenic, elastase substrate, ≥98%, powder
Synonym(s):
Nα-Benzoyl-DL-arginine p-nitroanilide hydrochloride, BANI, BAPNA
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About This Item
Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
Molecular Weight:
434.88
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
213-011-2
MDL number:
Beilstein/REAXYS Number:
4081878
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Product Name
Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%
InChI key
DEOKFPFLXFNAON-UHFFFAOYSA-N
InChI
1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H
SMILES string
Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O
assay
≥98%
form
powder
color
, white to light yellow to light brown
solubility
DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)
storage temp.
−20°C
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| assay ≥98% | assay >98% (TLC) | assay ≥98% (TLC) | assay ≥98% (TLC) |
| solubility DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow) | solubility acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow | solubility DMF: 50 mg/mL, clear, colorless to faintly yellow | solubility methanol: 20 mg/mL, clear, colorless to light yellow |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| color , white to light yellow to light brown | color - | color - | color - |
General description
Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (DL-BAPNA) is a chromogenic substrate for proteolytic enzymes such as trypsin, amidase, and balterobin. Hydrolysis of DL-BAPNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which can be detected by colorimetric analysis.
A chromogenic trypsin substrate.
Application
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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I Kurtovic et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 143(4), 432-440 (2006-02-07)
A trypsin fraction was isolated from the pyloric ceca of New Zealand farmed chinook salmon (Oncorhynchus tshawytscha) by ammonium sulfate fractionation, acetone precipitation and affinity chromatography. The chinook salmon enzyme hydrolyzed the trypsin-specific synthetic substrate benzoyl-DL-arginine-p-nitroanilide (DL-BAPNA), and was inhibited
Xixi Cai et al.
Molecules (Basel, Switzerland), 23(9) (2018-09-21)
Plant peptidase inhibitors play essential roles in the defense systems of plants. A trypsin inhibitor (PHTI) with a molecular mass of 20.5 kDa was isolated from the fresh roots of the medicinal herb, Pseudostellaria heterophylla. The purification process involved ammonium
Anju Singh et al.
Journal of biomolecular structure & dynamics, 1-14 (2017-09-21)
Regulatory regions in human genome, enriched in guanine-rich DNA sequences have the propensity to fold into G-quadruplex structures. On exploring the genome for search of G-tracts, it was interesting to find that promoter of Human Myosin Gene (MYH7) contains a
Guilherme M Zanatta et al.
Journal of periodontology, 77(3), 498-505 (2006-03-04)
The aim of the present study was to evaluate the clinical effects of one-stage periodontal debridement with an ultrasonic instrument, associated with 0.5% povidone (pvp)-iodine irrigation in patients with chronic periodontitis. Forty-five patients were randomly assigned into three groups: the
Nguyen Van Quan et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 27(5), 643-649 (2019-07-13)
This study was the first to detect the presence of the two compounds momilactone A (MA) and momilactone B (MB) in rice bran using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). By in vitro assays, both MA and MB exhibited potent inhibitory
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Why do the bottoms of the vials/bottles of product B4875-100MG (Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride) appear to have no bottom? How is the product contained within the vials despite this unusual appearance? Is this a packaging defect?
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