The 86C and 86R kits are intended for use with adherent cells. The same applies to SCR004. The APF kit, a fluorescence-based kit, would be more suitable for use if trying to detect alkaline phosphatase in culture media.
86C
Leukocyte Alkaline Phosphatase Kit
based on naphthol AS-BI and fast blue BB salt
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About This Item
UNSPSC Code:
12352106
NACRES:
NA.47
eCl@ss:
42010105
Pricing and availability is not currently available.
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
dilution
(for histology)
application(s)
hematology
histology
shipped in
wet ice
storage temp.
2-8°C
Application
Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast blue BB. The resulting insoluble diffuse, blue dye deposit indicates sites of alkaline phosphatase activity.
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Kit Components Only
Product No.
Description
- Citrate Solution (915) 50 mL
- FBB-Alkaline Solution (863) 10 mL
- Naphthol AS-BI Alkaline Solution (861) 10 mL
- Neutral Red Solution (N6264) 50 mL
- Sodium Nitrite Solution (914) 10 mL
signalword
Warning
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
wgk
WGK 3
Storage Class
10 - Combustible liquids
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Can the 86C or 86R kits be used to detect Alkaline Phosphatase in cell culture media?
1 answer-
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