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4-Nitrophenyl phosphate bis(tris) salt

Name: 4-Nitrophenyl phosphate bis(tris) salt
Brand: SIGMA

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

Synonym(s):

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

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About This Item

Linear Formula:

(C₆ H₄ N O₆ P)( C₄ H₁₂ N O₃)₂

CAS Number:

68189-42-4

Molecular Weight:

461.36

NACRES:

NA.32

PubChem Substance ID:

57652488

UNSPSC Code:

12352204

EC Number:

269-198-6

MDL number:

MFCD00037009

Beilstein/REAXYS Number:

1578142

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Properties

Product Name

4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, ≥97.0% (enzymatic)

Quality Segment

100

assay

≥97.0% (enzymatic)

form

powder or crystals

quality

for the determination of phosphatase

impurities

≤0.1% free nitrophenol, ≤6% water

solubility

H₂O: 0.1 g/mL, clear

storage temp.

2-8°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

Description

Application

4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.[1]

Biochem/physiol Actions

4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate,[2] preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs).[2] Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

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1 of 1

This Item	N3254	N3129	N3002
Sigma-Aldrich 73737 4-Nitrophenyl phosphate bis(tris) salt	Sigma-Aldrich N3254 4-Nitrophenyl phosphate di(tris) salt	Sigma-Aldrich N3129 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt	Sigma-Aldrich N3002 Bis(p-nitrophenyl) phosphate sodium salt
assay ≥97.0% (enzymatic)	assay ≥97% (enzymatic)	assay ≥97% anhydrous basis	assay ≥99% (TLC)
solubility H₂O: 0.1 g/mL, clear	solubility water: 50 mg/mL, clear, colorless to light yellow	solubility water: 10 mg/mL, clear, colorless to faintly yellow	solubility water: 20 mg/mL, clear to very slightly hazy
Quality Level 100	Quality Level 100	Quality Level 300	Quality Level 200
form powder or crystals	form powder	form powder	form powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
quality for the determination of phosphatase	quality -	quality -	quality -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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