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5(6)-Carboxynaphthofluorescein

Name: 5(6)-Carboxynaphthofluorescein
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Empirical Formula (Hill Notation):

C₂₉H₁₆O₇

CAS Number:

128724-35-6

Molecular Weight:

476.43

MDL number:

MFCD01862976

UNSPSC Code:

12352108

PubChem Substance ID:

329752223

NACRES:

NA.32

Pricing and availability is not currently available.

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Properties

product line

BioReagent

Quality Level

100

assay

≥90% (HPLC)

form

solid

fluorescence

λ_ex 512 nm; λ_em 567 nm (acid/neutral), λ_ex 598 nm; λ_em 668 nm (basic)

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc5cc(O)ccc5c4Oc6c3ccc7cc(O)ccc67.OC(=O)c8ccc9C(=O)OC%10(c%11ccc%12cc(O)ccc%12c%11Oc%13c%10ccc%14cc(O)ccc%13%14)c9c8

InChI

1S/2C29H16O7/c30-17-4-6-19-14(11-17)1-9-23-25(19)35-26-20-7-5-18(31)12-15(20)2-10-24(26)29(23)22-8-3-16(27(32)33)13-21(22)28(34)36-29;30-17-4-7-19-14(11-17)2-9-22-25(19)35-26-20-8-5-18(31)12-15(20)3-10-23(26)29(22)24-13-16(27(32)33)1-6-21(24)28(34)36-29/h2*1-13,30-31H,(H,32,33)

InChI key

ANUHNEJUELPITC-UHFFFAOYSA-N

Description

Application

5(6)-Carboxynaphthofluorescein (CNF) may be used as an intravesicular pH-sensitive probe for the detection of activity, selectivity and cooperativity of ion channels such as gramicidin A.

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This Item	46935	46940	46950
Sigma-Aldrich 21932 5(6)-Carboxynaphthofluorescein	Sigma-Aldrich 46935 6-[Fluorescein-5(6)-carboxamido]hexanoic acid	Sigma-Aldrich 46940 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester	Sigma-Aldrich 46950 Fluorescein 5(6)-isothiocyanate
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence
fluorescence λ_ex 512 nm; λ_em 567 nm (acid/neutral), λ_ex 598 nm; λ_em 668 nm (basic)	fluorescence λ_ex 491 nm; λ_em 515 nm in 0.1 M Tris pH 9.0	fluorescence λ_ex 490 nm; λ_em 514 nm (pH 8.2), λ_ex 491 nm; λ_em 515 nm in 0.1 M Tris pH 9.0	fluorescence λ_ex 492 nm; λ_em 518 nm in 0.1 M phosphate pH 8.0
form solid	form solid	form solid	form powder
assay ≥90% (HPLC)	assay ≥90% (HPCE)	assay ≥75% (HPLC)	assay ≥90% (HPLC)
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 100
product line BioReagent	product line -	product line -	product line BioReagent

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Array biosensor based on enzyme kinetics monitoring by fluorescence spectroscopy: application for neurotoxins detection.

M Ramanathan et al.

Biosensors & bioelectronics, 22(12), 3001-3007 (2007-02-03)

The aim of the present work is to develop an evanescence wave array biosensor exploiting the "kinetic" approach of enzymatic reaction and further detection of the reaction products via pH sensitive fluorophore reporter. To demonstrate the feasibility of this approach

Symbiodinium mitigate the combined effects of hypoxia and acidification on a noncalcifying cnidarian.

Shannon G Klein et al.

Global change biology, 23(9), 3690-3703 (2017-04-09)

Anthropogenic nutrient inputs enhance microbial respiration within many coastal ecosystems, driving concurrent hypoxia and acidification. During photosynthesis, Symbiodinium spp., the microalgal endosymbionts of cnidarians and other marine phyla, produce O

High-performance fiber-optic pH microsensors for practical physiological measurements using a dual-emission sensitive dye.

A Song et al.

Analytical chemistry, 69(5), 863-867 (1997-03-01)

A fast and durable ratiometric pH microoptode that is highly accurate, precise, sensitive, reversible, and reproducible over the physiological ranges of pH, ionic strength, and temperature has been developed. The sensing site consists of 5 (and 6)-carboxynaphthofluorescein (CNF) entrapped in