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Atto 532

Name: Atto 532
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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About This Item

UNSPSC Code:

12352108

NACRES:

NA.25

MDL number:

MFCD05865400

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Properties

product line

BioReagent

Quality Level

100

assay

≥90% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λ_ex 533 nm; λ_em 560 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Description

General description

Atto 532 is a new label with high molecular absorption (115,000) and quantum yield (0.90) as well as sufficient Stoke′s shift between excitation and emission maximum. It is optimized for excitation with frequency doubled Nd:YAG-Laser, and is characterized by high photostability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 532 has been conjugated with the secondary antibodies for STED (stimulated emission depletion) microscopy and immunofluorescence studies.[1]

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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This Item	41051	88793	40692
Sigma-Aldrich 06699 Atto 532	Sigma-Aldrich 41051 Atto 488	Sigma-Aldrich 88793 Atto 532 NHS ester	Sigma-Aldrich 40692 Atto 532 DOPE
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence
fluorescence λ_ex 533 nm; λ_em 560 nm in 0.1 M phosphate pH 7.0	fluorescence -	fluorescence -	fluorescence λ_ex 537 nm; λ_em 559 nm±5 nm in ethanol
assay ≥90% (HPCE)	assay ≥90% (HPLC)	assay ≥90% (HPLC), ≥90% (degree of coupling)	assay ≥80.0% (HPCE)
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
product line BioReagent	product line BioReagent	product line BioReagent	product line -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

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