The ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage.
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UNSPSC Code:
12352207
NACRES:
NA.81
eCl@ss:
32160801
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ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A Human Neural Progenitor Cells. Using ENStem-A Neuronal Differentiation Medium, relatively pure populations of neuronal cells (>90% bIII-tubulin positive) are obtained. Very low levels of astrocytes (<0.5% GFAP positive) are detected. Sufficient medium is supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume.
Application
Cell culture protocols for use with ENStem-A Human Neural Progenitor Cells are available in the datasheets that accompany the ENStem-A Human Neural Progenitor Expansion Kit (Part No. SCR055).
The ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage.
Preparation Note
ENStem-A Neuronal Differentiation Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the medium. Thawed medium should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.
Analysis Note
Each lot of media is tested for the optimal differentiation of ENStem-A Human Neural Progenitor Cells to a neuronal lineage. Sterility Testing: Negative
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
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As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.