The In Vitro Vascular Permeability Assay is performed in a 96-well receiver plate with 96 cell culture inserts connected in a single tray unit. Because the inserts are interconnected, the risk of cross-contamination is significantly increased if only a subset of wells is used at a time. In addition, the collagen coating of unused wells may be compromised during incubation. For these reasons, we strongly recommend utilizing all inserts within a single experiment to ensure assay integrity and reproducibility.
ECM642
In Vitro Vascular Permeability Assay (96-well)
This In Vitro Vascular Permeability Assay kit employs a 96-well plate, and provides an efficient system for evaluating the effects of chemicals & drug compounds on endothelial cell adsorption, transport & permeability.
Synonym(s):
Endothelial cell model for permeability assay
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.32
eCl@ss:
32161000
Pricing and availability is not currently available.
Quality Segment
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Application
Research Category
Cell Structure
Cell Structure
The Millipore® In Vitro Vascular Permeability Assay kit provides an efficient system for evaluating the effects of chemicals and drug compounds on endothelial cell adsorption, transport, and permeability. It is ideal for measuring compounds that may disrupt or protect an endothelial monolayer. Each In Vitro Vascular Permeability Assay Kit contains sufficient reagents for the evaluation of 96 samples. The In Vitro Vascular Permeability Assay Kit is intended for research use only, not for diagnostic or therapeutic applications.
Preparation Note
Store kit materials at 2-8°C; use within 4 months from date of receipt. Do not freeze.
Other Notes
96-Well Permeability Insert Assembly, Collagen-coated: One 96-well receiver tray containing 96 connected porous cell culture inserts pre-coated with type I rat-tail collagen.
Receiver Tray, 96-Well: Two 96-well plates.
FITC-Dextran Solution: Two vials containing 250 µL.
Cell Stain: One bottle containing 10 mL.
Black Plate, 96-Well Opaque: One 96-well plate.
Receiver Tray, 96-Well: Two 96-well plates.
FITC-Dextran Solution: Two vials containing 250 µL.
Cell Stain: One bottle containing 10 mL.
Black Plate, 96-Well Opaque: One 96-well plate.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10-13 - German Storage Class 10 to 13
wgk
WGK 2
Certificates of Analysis (COA)
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Articles
Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.
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Is it possible to separate inserts and use them in different experiments? Or should the whole plate be used at once?
1 answer-
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Could you provide information on the pore size of EMD Millipore ECM644?
1 answer-
The pore size of ECM644 is 1.0 µm. It is a 6.5 mm diameter filter with a filtration area of 0.33 cm², which serves as the growth surface area.
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Could you provide the pore size information for ECM642?
1 answer-
The ECM 642 filter has a pore size of 1.0 µm, a filter diameter of 0.4 mm, and a filtration area of 0.12 cm² (growth surface area).
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