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53702

Pronase® Protease, Streptomyces griseus

Synonym(s):

Pronase® powder

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
eCl@ss:
32160410
Specific activity:
≥45,000 proteolytic units/g dry wt
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description

RTECS - UK9595000

Quality Level

form

lyophilized

specific activity

≥45,000 proteolytic units/g dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic)

solubility

water: 10 mg/mL

shipped in

ambient

storage temp.

2-8°C

General description

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
Note: 1 KU = 1000 units.

Physical form

Lyophilized from a solution containing calcium acetate as a stabilizer.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.

Other Notes

Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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This Item
537088P0652P8811
specific activity

≥45,000 proteolytic units/g dry wt

specific activity

≥70,000 proteolytic units/g dry wt

specific activity

≥15 units/mg solid

specific activity

≥3.5 units/mg solid

form

lyophilized

form

lyophilized

form

powder

form

powder

solubility

water: 10 mg/mL

solubility

water: 10 mg/mL

solubility

-

solubility

-

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze

storage condition

-

storage condition

-


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The preparation and characterization of cell walls and the preparation of flagella of Vibrio fetus.
R F Keeler et al.
Journal of general microbiology, 43(3), 439-454 (1966-06-01)
T E Davis et al.
Infection and immunity, 15(3), 978-987 (1977-03-01)
Enzymes capable of hydrolyzing cell walls of Blastomyces dermatitidis and chemotypes I and II of Histoplasma capsulatum were prepared in the laboratory or obtained from commercial sources. They included chitinases, beta-1,3-glucanases, beta-1,6-glucanase, and Pronase. Monosaccharides and disaccharides of glucose released
Yu Zhang et al.
Frontiers in molecular neuroscience, 11, 222-222 (2018-07-14)
During development, immature blood vessel networks remodel to form a simplified and efficient vasculature to meet the demand for oxygen and nutrients, and this remodeling process is mainly achieved via the pruning of existing vessels. It has already known that



Questions

  1. What is the difference between these two products of yours? first: SKU no. 10165921001 second SKU no. 53702-10KU

    1 answer
    1. The manufacturer/tradename of PRON-RO (10165921001) is Roche where as the manufacturer/tradename of 53702 is Calbiochem®. The SKU and pack sizes are also different for both.
      Answer Source:
      1.https://www.sigmaaldrich.com/IN/en/product/roche/pron-ro
      2. https://www.sigmaaldrich.com/IN/en/product/mm/53702

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