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Anti-MAP2K1/MEK1 Antibody

Name: Anti-MAP2K1/MEK1 Antibody
Brand: MM

Upstate, rabbit polyclonal

Synonym(s):

Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Clone:

polyclonal

Species reactivity:

mouse, rat, human

Application:

IP, WB

Citations:

Pricing and availability is not currently available.

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Properties

Product Name

Anti-MEK1 Antibody, Upstate^®, from rabbit

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate^®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_002755

UniProt accession no.

Q02750

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAP2K1(5604)

Description

General description

45kDa

Immunogen

Peptide corresponding to amino acids 2-18 (PKKKPTPIQLNPAPDGS) of human MEK1.

Application

Research Category
Signaling

Research Sub Category
MAP Kinases

This Anti-MEK1 Antibody is validated for use in IP, WB for the detection of MEK1.

Biochem/physiol Actions

MEK1

Predicted to cross-react with rabbit based on sequence homology.

Physical form

70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol.

Format: Purified

Protein A purified

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.

Routinely evaluated by immunoblot on RIPA lysates from A431 and 3T3/A31 cells.

Other Notes

Replaces: 04-376

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M5795	WH0005604M1	SAB2500959
Sigma-Aldrich 07-641 Anti-MEK1 Antibody	Sigma-Aldrich M5795 Anti-MAP Kinase Kinase (MEK, MAPKK) antibody produced in rabbit	Sigma-Aldrich WH0005604M1 Monoclonal Anti-MAP2K1 antibody produced in mouse	Sigma-Aldrich SAB2500959 Anti-SMEK1/KIAA2010 antibody produced in goat
biological source rabbit	biological source rabbit	biological source mouse	biological source goat
species reactivity mouse, rat, human	species reactivity mouse, human, rat	species reactivity human	species reactivity human, canine, mouse, rat
clone polyclonal	clone polyclonal	clone 1B5, monoclonal	clone polyclonal
antibody form purified immunoglobulin	antibody form whole antiserum	antibody form purified immunoglobulin	antibody form affinity isolated antibody
UniProt accession no. Q02750	UniProt accession no. P36507	UniProt accession no. Q02750	UniProt accession no. -
technique(s) immunoprecipitation (IP): suitable, western blot: suitable	technique(s) microarray: suitable, western blot: 1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast lysate	technique(s) indirect ELISA: suitable, western blot: 1-5 μg/mL	technique(s) indirect ELISA: suitable, western blot: suitable

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

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