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04-478

Anti-phospho-STAT1 (Ser 727) Antibody, clone 12C5

clone 12C5, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
12C5, monoclonal
Species reactivity:
human
Application:
ELISA, WB
Citations:
10
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biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

12C5, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_007315.2, NM_139266.1

UniProt accession no.

P42224

shipped in

wet ice

target post-translational modification

phosphorylation (pSer727)

Gene Information

human ... STAT1(6772)

General description

92 kDa

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Serine727 on phosphorylated STAT1

Application

Anti-phospho-STAT1 (Ser 727) Antibody, clone 12C5 is a Mouse Monoclonal Antibody for detection of phospho-STAT1 (Ser 727) also known as signal transducer & activator of transcription 1 & has been tested in ELISA & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Biochem/physiol Actions

Recognizes phosphorylated domain of STAT1 on Serine727

Physical form

Format: Purified
In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose, and 50% glycerol.
Subsequent thiophilic adsorption and size exclusion chromatography

Preparation Note

2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
SAB5700330SAB570184105-1064
Anti-phospho-STAT1 (Ser 727) Antibody, clone 12C5 clone 12C5, Upstate®, from mouse

Sigma-Aldrich

04-478

Anti-phospho-STAT1 (Ser 727) Antibody, clone 12C5

Anti-Phospho-STAT1-S727 antibody produced in rabbit

Sigma-Aldrich

SAB5700330

Anti-Phospho-STAT1-S727 antibody produced in rabbit

Anti-Phospho-STAT1-S727 antibody produced in rabbit

Sigma-Aldrich

SAB5701841

Anti-Phospho-STAT1-S727 antibody produced in rabbit

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 clone 5C9.2, from mouse

Sigma-Aldrich

05-1064

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

clone

12C5, monoclonal

clone

polyclonal

clone

monoclonal

clone

5C9.2, monoclonal

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

-

antibody form

purified immunoglobulin

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

human

species reactivity

rat, mouse, human

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry: 1:50-1:100, immunoprecipitation (IP): 1:50-1:100, western blot: 1:500-1:2000

technique(s)

western blot: 1:500-1:2000

technique(s)

western blot: suitable

UniProt accession no.

P42224

UniProt accession no.

P42224

UniProt accession no.

P42224

UniProt accession no.

P42224

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Storage Class

10 - Combustible liquids

wgk

WGK 2

Certificates of Analysis (COA)

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JAK/STAT Signaling Research Focus

The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia.

H Nguyen et al.
The Journal of biological chemistry, 276(36), 33361-33368 (2001-07-05)
STAT1 must be phosphorylated on serine 727 to be fully active in transcription. We show that phosphatidylinositol 3-kinase (PI3K) and its effector kinase Akt play an important role in the serine phosphorylation of STAT1 and in the activation of gene
Christopher P Gayer et al.
The Journal of biological chemistry, 284(4), 2001-2011 (2008-12-03)
The intestinal epithelium is repetitively deformed by shear, peristalsis, and villous motility. Such repetitive deformation stimulates the proliferation of intestinal epithelial cells on collagen or laminin substrates via ERK, but the upstream mediators of this effect are poorly understood. We
Rico K H Lo et al.
Cellular signalling, 18(3), 307-317 (2005-06-28)
The ability of the human prostacyclin receptor (hIP) to regulate the activities of signal transducers and activators of transcription (STATs) has not yet been documented. In the present study, we have delineated the mechanism by which hIP induces STAT3 phosphorylations
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