The concentration for product T6025 is 40 mM Tris acetate with 1 mM EDTA at approximately pH 8.3. This solution, prepared with 18 megohm water and 0.2 μm filtration, is considered a 1X working solution.
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無菌
0.2 μm filtered
品質等級
產品線
BioReagent
形狀
working solution
技術
electrophoresis: suitable
雜質
DNase, RNase, Protease, none detected
適合性
suitable for electrophoresis
suitable for gel electrophoresis (after dilution to working concentration)
應用
diagnostic assay manufacturing
SMILES 字串
CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
InChI 密鑰
HGEVZDLYZYVYHD-UHFFFAOYSA-N
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應用
TAE流动缓冲液是DNA琼脂糖凝胶电泳最常用的缓冲液，也可用于非变性RNA琼脂糖凝胶电泳。 双链DNA在TAE中比在其他缓冲液中运行更快，但也可能在延长的电泳过程中耗尽。 在延长的电泳过程中，缓冲液循环或缓冲液更换可以弥补较低的缓冲容量。 浓缩TAE缓冲液稀释后得到1× TAE缓冲液，加入40 mM醋酸三酯和1 mM EDTA，pH 8.3。 1× TAE缓冲液用于琼脂糖凝胶和作为流动缓冲液。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。
其他說明
40 mM三醋酸盐，pH 约为8.3，包含1 mM EDTA。
準備報告
用18 兆欧水制备的溶液。
由经过生物技术性能认证的Trizma碱(T6066)和分子生物学试剂EDTA二钠盐(E5134)制备而成。溶液也含有醋酸(A6283)；粉状混合物含Trizma醋酸(T1258)。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Could you provide the concentration of product T6025 and clarify if it is considered 50X, 10X, or 1X?
1 answer-
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