The reconstituted protease inhibitor solution may be stored at 2-8 °C for at least 2 weeks. Long term solution stability studies at -20°C have not been performed, however 10X solutions - 10mL per tablet - will be stable for at least 1 month in frozen aliquots. Avoid repeated freeze/thaw cycles.
推荐产品
产品名称
SIGMAFAST ™蛋白酶抑制剂混合物片剂，无EDTA, for use in purification of Histidine-tagged proteins
生物来源
bovine lung
质量水平
表单
tablet
储存温度
2-8°C
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一般描述
SIGMAFAST™蛋白酶抑制剂混合液片（不含EDTA）是一种水溶性片剂配方，旨在抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。该片剂不含强金属螯合剂，如EDTA，并与固定化金属亲和层析（IMAC）样品处理兼容。该片剂还含有独特的专利配方，包括胃酶抑素A盐，以提供额外的保护。
特异性
抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。
应用
SIGMAFAST™蛋白酶抑制剂混合液片具有广谱特异性，是通用的蛋白酶抑制剂混合物，适于组氨酸标记蛋白的纯化。片剂可加入细胞裂解缓冲液。还经过优化和测试，可与HIS-Select®介质配合使用。
特点和优势
EDTA-Free： 不含可能干扰IMAC处理的强金属螯合剂，如EDTA。
胃酶抑素A：含有独特的专有配方（包括胃抑素A盐），可提供片剂形式通常无法提供的额外保护作用。
广泛特异性：抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶，为蛋白质样品提供广泛的保护。
高产率：一片可制成100 mL混合液，推荐用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
长期稳定性：该片剂在2–8 °C温度下至少可稳定保存2年。复溶的蛋白酶抑制剂溶液（1´或10´）在2–8 °C下至少可稳定保存2周。
胃酶抑素A：含有独特的专有配方（包括胃抑素A盐），可提供片剂形式通常无法提供的额外保护作用。
广泛特异性：抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶，为蛋白质样品提供广泛的保护。
高产率：一片可制成100 mL混合液，推荐用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
长期稳定性：该片剂在2–8 °C温度下至少可稳定保存2年。复溶的蛋白酶抑制剂溶液（1´或10´）在2–8 °C下至少可稳定保存2周。
组分
AEBSF
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽
数量
一片可制备100 mL混合液。 推荐将一片用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
外形
片剂是可溶于水的
储存及稳定性
该片剂在2-8°C温度下至少可稳定保存2年。重悬的蛋白酶抑制剂溶液（1´或10´）在2–8°C下至少可稳定保存2周。
法律信息
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
相关产品
产品编号
说明
价格
通常也和此产品一起购买
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Skin Corr. 1A
储存分类代码
8A - Combustible corrosive hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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商品
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
相关内容
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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Can the reconstituted protease inhibitor cocktail solution be stored at -20°C and for how long?
1 answer-
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How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
1 answer-
You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use. Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
1 answer-
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
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Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
1 answer-
Yes. Several examples of published papers that cite the use of Product S8830 include these:1. Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies. Clinica Chimica Acta, 412(11-12), 1008-1011 (2011). Use was with blood at 20 μL cocktail per 1 mL of blood.2. Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011).
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What is the difference between Protease Inhibitors P8849 and S8830?
1 answer-
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
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