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Merck

S0146

Sigma-Aldrich

Schneider 昆虫培养基

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

别名:

Schneider’s Drosophila Medium

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100 ML
$45.20
500 ML
$124.00
1 L
$265.00
6 X 500 ML
$615.00
6 X 1 L
$1,370.00

$45.20


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100 ML
$45.20
500 ML
$124.00
1 L
$265.00
6 X 500 ML
$615.00
6 X 1 L
$1,370.00

About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

$45.20


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产品名称

Schneider 昆虫培养基, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | insect: suitable

適合性

suitable for culture of other dipteran cell lines (Originally developed for the culture of Drosophila cells)

成分

NaHCO3: 0.4 g/L
glucose: 2 g/L (Dextro)
L-glutamine: 1.8 g/L

運輸包裝

ambient

儲存溫度

2-8°C

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應用

Schneider′s昆虫培养基已用于:
  • 培养果蝇神经元细胞株(BG3-c2细胞)[1]
  • 培养硕大利什曼原虫(Leishmania major),用于原虫生长抑制试验[2]
  • 接种纯化动合子和黑腹果蝇(Drosophila melanogaster)S2细胞,研究阿维菌素化合物对细胞发育的影响并促进卵母细胞发育[3]

原为果蝇培养而开发;也适用于其他双翅类细胞系的培养。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

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Anna Franz et al.
The Journal of cell biology, 203(5), 785-799 (2013-12-04)
CP110 is a conserved centriole protein implicated in the regulation of cell division, centriole duplication, and centriole length and in the suppression of ciliogenesis. Surprisingly, we report that mutant flies lacking CP110 (CP110Δ) were viable and fertile and had no
Eva Wegel et al.
Scientific reports, 6, 27290-27290 (2016-06-07)
Many biological questions require fluorescence microscopy with a resolution beyond the diffraction limit of light. Super-resolution methods such as Structured Illumination Microscopy (SIM), STimulated Emission Depletion (STED) microscopy and Single Molecule Localisation Microscopy (SMLM) enable an increase in image resolution
Amanda R Brock et al.
Genetics, 206(3), 1505-1520 (2017-05-18)
Regeneration is a complex process that requires an organism to recognize and repair tissue damage, as well as grow and pattern new tissue. Here, we describe a genetic screen to identify novel regulators of regeneration. We ablated the
Jeffrey S Dason et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(38), 23286-23291 (2019-06-20)
Painful or threatening experiences trigger escape responses that are guided by nociceptive neuronal circuitry. Although some components of this circuitry are known and conserved across animals, how this circuitry is regulated at the genetic and developmental levels is mostly unknown.
Amit Sinha et al.
Genome biology and evolution, 11(3), 706-720 (2019-02-05)
Wolbachia, an alpha-proteobacterium closely related to Rickettsia, is a maternally transmitted, intracellular symbiont of arthropods and nematodes. Aedes albopictus mosquitoes are naturally infected with Wolbachia strains wAlbA and wAlbB. Cell line Aa23 established from Ae. albopictus embryos retains only wAlbB

Questions

  1. Is it possible to freeze this medium ? I'll need to use only a few millilitres for each dissections of fly brains so it'll be great if I could freeze it in smaller containers to extend the shelf life period.

    1 answer
    1. This medium has not been evaluated for freezer storage. The thawing process may cause precipitation of some salts. The end user may determine the suitability experimentally by freezing a small test volume. It is advisable to thaw the product slowly to minimize the chance of precipitation. Thaw the sample slowly, overnight in a refrigerator., if possible It will very likely be suitable if the resulting thawed medium is clear, colorless, and free of particulate matter. Do not add serum or supplements prior to freezing the test sample.

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