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一般描述
Trypsin Profile IGD Kit 能够对多达 100 个切除的蛋白点进行快速、高效和完全的凝胶内胰蛋白酶消化。消化后的蛋白质可用于 MALDI-MS（基质辅助激光解吸/电离质谱法），无需额外制备。由于 Trypsin Profile IGD Kit 含有蛋白质组学级别的胰蛋白酶，在 MALDI 光谱中观察到较高的序列覆盖率和较少的模棱两可的自溶峰。
通过赖氨酸的ε-氨基的还原甲基化对胰蛋白酶进行化学修饰，减少自溶，使自溶片段最小化。此外，还经过 TPCK（对甲苯磺酰基苯丙氨酰基氯甲基酮）处理，去除残留的糜蛋白酶活性，然后通过亲和层析进一步纯化，得到了适合蛋白质组学工作的高纯度胰蛋白酶。
通过赖氨酸的ε-氨基的还原甲基化对胰蛋白酶进行化学修饰，减少自溶，使自溶片段最小化。此外，还经过 TPCK（对甲苯磺酰基苯丙氨酰基氯甲基酮）处理，去除残留的糜蛋白酶活性，然后通过亲和层析进一步纯化，得到了适合蛋白质组学工作的高纯度胰蛋白酶。
特点和优势
- 相比其它凝胶中消化试剂盒来说可更快速地完成脱色
- 包含对感兴趣蛋白/多肽进行脱色、消化及提取所需的所有试剂
- 兼容使用Coomassie, SYPRO® Orange, SYPRO Ruby, and ProteoSilver™等进行染色的凝胶
- 所得样品可即用于MALDI-MS或HPLC-MS分析
法律信息
ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a registered trademark of Life Technologies
仅试剂盒组分
产品编号
说明
- Destaining Solution Reconstituted to 75
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
3 - Flammable liquids
闪点(°F)
35.6 °F - closed cup
闪点(°C)
2.0 °C - closed cup
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Protein modifications are crucial for disease study. Analysis methods are key.
One of the most important aspects of our ultra-pure MALDI matrix substances is their ability to dissolve rapidly and completely; a brief vortex mixing is typically sufficient.
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One of the most important aspects of our ultra-pure MALDI matrix substances is their ability to dissolve rapidly and completely; a brief vortex mixing is typically sufficient.
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